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  4. Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His)

Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His)

Cat. No.: HY-P77078
COA Handling Instructions

Mast Cell Protease-1 (MCPT-1) displays chymotrypsin-like enzymatic activity. Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Mast Cell Protease-1/MCPT-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is 228 a.a., with molecular weight of 32-34 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Mast Cell Protease-1 (MCPT-1) displays chymotrypsin-like enzymatic activity. Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Mast Cell Protease-1/MCPT-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is 228 a.a., with molecular weight of 32-34 kDa.

Background

Mast Cell Protease-1 (MCPT-1) exhibits a chymotrypsin-like enzymatic activity.

Biological Activity

Measured by its ability to cleave Suc-Ala-Ala-Pro-Phe-AMC. The specific activity is 51.502 pmol/min/μg, as measured under the described conditions.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P11034 (E19-K246)

Gene ID

17224  [NCBI]

Molecular Construction
N-term
MCPT-1 (E19-K246)
Accession # P11034
His
C-term
Synonyms
Mast cell protease 1; mMCP-1; Mcpt1
AA Sequence

EEIIGGVEARPHSRPYMAHLKIITDRGSEDRCGGFLIAPQFVLTAAHCKGREITVTLGAHDVSKSESTQQRIKVEKQIIHKNYNVSFNLYDIMLLKLEEKAELTPTVDVIPLPGPSDFIDPGKMCWTAGWGKTGEKEPTSETLREVELRIMDKEACKMYKHYDYNFQVCVGSSTKLKTAYMGDSGGPLLCAGVAHGIVSYGDSHGKPPAVFTRISAYVPWIKTVINGK

Molecular Weight

Approximately 30-32 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His)
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HY-P77078
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