Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Mast Cell Protease-1 (MCPT-1) displays chymotrypsin-like enzymatic activity.Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Mast Cell Protease-1/MCPT-1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Mast Cell Protease-1 (MCPT-1) displays chymotrypsin-like enzymatic activity.Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Mast Cell Protease-1/MCPT-1 protein, expressed by HEK293 , with C-His labeled tag.
Background
Mast Cell Protease-1 (MCPT-1) exhibits a chymotrypsin-like enzymatic activity.
Verified Bioactivity
Measured by its ability to cleave Suc-Ala-Ala-Pro-Phe-AMC. The specific activity is 51.502 pmol/min/μg, as measured under the described conditions. (Activation description: The proenzyme needs to be activated by Mouse Cathepsin C (HY-P704018) and Heparin for an activated form.)
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-6*His
-
Accession
P11034 (E19-K246)
-
Gene ID17224 [NCBI]
-
Molecular Construction
-
N-term
-
MCPT-1 (E19-K246)
Accession # P11034 -
His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
CPT1B; Carnitine O-Palmitoyltransferase 1B; Carnitine Palmitoyltransferase 1B; CPTI-M; CPT1-M; KIAA1670; Carnitine O-Palmitoyltransferase 1, Muscle Isoform; MCCPT1; M-CPT1; CPT1M; Carnitine O-Palmitoyltransferase I, Muscle Isoform; MCPT1; Carnitine Palmit
-
AA Sequence
EEIIGGVEARPHSRPYMAHLKIITDRGSEDRCGGFLIAPQFVLTAAHCKGREITVTLGAHDVSKSESTQQRIKVEKQIIHKNYNVSFNLYDIMLLKLEEKAELTPTVDVIPLPGPSDFIDPGKMCWTAGWGKTGEKEPTSETLREVELRIMDKEACKMYKHYDYNFQVCVGSSTKLKTAYMGDSGGPLLCAGVAHGIVSYGDSHGKPPAVFTRISAYVPWIKTVINGK
-
Molecular Weight
Approximately 32-34 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (236 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
