MAT1A Protein, Human (His)
MAT1A Protein, Human (His) is the recombinant human-derived MAT1A protein, expressed by E. coli, with N-10*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
MAT1A Protein, Human (His) is the recombinant human-derived MAT1A protein, expressed by E. coli, with N-10*His tag.
MAT1A is an enzyme that catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The reaction involves two steps, both catalyzed by the same enzyme: the formation of S-adenosylmethionine (AdoMet) and triphosphate, followed by the hydrolysis of the triphosphate.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His
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Accession
Q00266 (M1-F395)
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Gene ID4143
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Molecular Construction
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N-term
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10*His
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MAT1A (M1-F395)
Accession # Q00266 -
C-term
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Protein Length
Full Length
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Synonyms
MAT1A; Methionine Adenosyltransferase I, Alpha; Methionine Adenosyltransferase 1A; Methionine Adenosyltransferase 1; MAT-I/III; AdoMet Synthase 1; MATA1; MAT 1; S-Adenosylmethionine Synthase Isoform Type-1; S-Adenosylmethionine Synthetase; Methionine Aden
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AA Sequence
MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF
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Predicted Molecular Mass
47.3 kDa
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Molecular Weight
Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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