MD-2/LY96 Protein, Human (His)
Based on 1 Customer Validation
Myeloid differentiation factor-2 (MD2) (LY96 gene) is a small glycoprotein expressed by macrophages and dendritic cells. MD2 functions as a co-receptor for toll-like receptor (TLR) 4 and is required for its activation. Recognition of LPS by TLR4 requires MD-2 since most of the lipid chains of LPS interact with a hydrophobic pocket in MD-2. MD2 confers invasiveness by activating MAPK and NF-kB signaling pathways and inducing epithelial-mesenchymal transition. MD-2/LY96 Protein, Human (His) is the recombinant human-derived MD-2/LY96 protein, expressed by E. coli , with C-10*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Myeloid differentiation factor-2 (MD2) (LY96 gene) is a small glycoprotein expressed by macrophages and dendritic cells. MD2 functions as a co-receptor for toll-like receptor (TLR) 4 and is required for its activation. Recognition of LPS by TLR4 requires MD-2 since most of the lipid chains of LPS interact with a hydrophobic pocket in MD-2. MD2 confers invasiveness by activating MAPK and NF-kB signaling pathways and inducing epithelial-mesenchymal transition[1][2]. MD-2/LY96 Protein, Human (His) is the recombinant human-derived MD-2/LY96 protein, expressed by E. coli , with C-10*His labeled tag.
Myeloid differentiation factor-2 (MD2) plays a critical role in metastasis and CaP progression. MD2 is an essential factor in the tumor microenvironment allowing CaP cells to acquire metastatic traits[1].
Recognition of LPS by TLR4 requires MD-2 since most of the lipid chains of LPS interact with a hydrophobic pocket in MD-2. Engagement of LPS with the TLR4/MD-2 complex triggers dimerization of two TLR4/MD-2 complexes, which leads to the recruitment of two major adaptor molecules, MyD88 and TRIF, and the activation of intracellular signalling pathways (i.e. MAPKs and NF-κB) for the production of pro-inflammatory cytokines[2].
Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 (HY-P73586) is Immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated recombinant human MD-2. The ED50 for this effect is 0.1453 μg/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-10*His
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Accession
Q9Y6Y9-1/BAA78717 (E17-N160)
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Molecular Construction
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N-term
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LY96 (E17-N160)
Accession # Q9Y6Y9-1/BAA78717 -
10*His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
LY96; Ly-96; Lymphocyte Antigen 96; MD2; MD-2; Myeloid Differentiation Protein-2; Protein MD-2; MD-2 Mimetic Protein Variant; ESOP-1; ESOP1
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AA Sequence
EAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN
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Predicted Molecular Mass
18 kDa
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Molecular Weight
Approximately 19 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 30% Acetonitrile, pH 2.5.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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