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IL-17A Protein, Human

6 Cited Publications
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Based on 6 publication(s) in Google Scholar

IL-17A is a homodimeric cytokine and plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides, cytokines, chemokines, and matrix metalloproteinases. IL-17A Protein, Human is a recombinant human IL-17A protein and is expressed in E. coli. It consists of 137 amino acids (I19-A155).

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • IL-17A Protein, Human Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

IL-17A is a homodimeric cytokine and plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides, cytokines, chemokines, and matrix metalloproteinases[1][2]. IL-17A Protein, Human is a recombinant human IL-17A protein and is expressed in E. coli. It consists of 137 amino acids (I19-A155).

Background

Interleukin-17A (IL-17A), also known as CTLA-8, belongs to the IL-17 cytokine family. IL-17A is expressed in memory Th17 cells and is a product of memory CD4+ T cells. IL-17A is also produced by a wide variety of immune cells, including CD8+ T cells, γδT cells, natural killer T (NKT) cells, monocytes, and neutrophils[1][2][3].
The human IL-17A shares 63.23% amino acid sequence identity with mouse and 61.90% identity with rat.
IL-17A plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides (defensins and S100 proteins), cytokines (IL-6, G-CSF, and GM-CSF), chemokines (CXCL1, CXCL5, IL-8, CCL2, and CCL7), and matrix metalloproteinases (MMP1, MMP3, and MMP13). IL-17A is detrimental in viral infection through promoting neutrophilic inflammation. IL-17A is a homodimeric cytokine and shares similar biological activities with IL-17F. IL-17A binds to IL-17RA with high affinity, and IL-17RA is required for the biological activity of IL-17A. In tumorigenesis, IL-17A recruits myeloid derived suppressor cells (MDSCs) to dampen anti-tumor immunity. IL-17A also enhances tumor growth in vivo through the induction of IL-6[1][2].
IL-17A can be used for the research of autoimmune diseases, infection and cancer[1][4].

In Vitro

IL-17A (100 ng/mL; 24 h) inhibits the expression of thymic stromal lymphopoietin (TSLP) in human primary keratinocytes[5].
IL-17A (0.1–100 ng/mL; 0-24 h) significantly potentiates TNF-α-induced IL-8 protein secretion and gene expression in a concentration- and time-dependent manner[6].

Verified Bioactivity

1.Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells and the ED50 is 1-7 ng/mL.
2.Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is ≤3.340 ng/mL. Corresponding to a specific activity is ≥2.99 × 105 U/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q16552 (G24-A155)

  • Gene ID
    3605  [NCBI]
  • Molecular Construction
    • N-term
    • IL-17A (G24-A155)
      Accession # Q16552
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IL17A; Cytotoxic T-Lymphocyte-Associated Protein 8; Prev. CTLA8; Interleukin-17A; Prev. IL17; CTLA-8; IL-17A; Interleukin-17; IL-17; ILA17; Interleukin 17 (Cytotoxic T-Lymphocyte-Associated Serine Esterase 8); Interleukin 17A; Cytotoxic T-Lymphocyte-Assoc

  • AA Sequence

    GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA

  • Predicted Molecular Mass

    15.3 kDa

  • Molecular Weight

    Approximately 16 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 500 mM arginine, pH 8.0.
3.Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, pH 2.5.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Chen K, et al. Interluekin-17A (IL17A). Gene. 2017 May 30;614:8-14. [Content Brief]

[2]. Iwakura Y, et al. The roles of IL-17A in inflammatory immune responses and host defense against pathogens. Immunol Rev. 2008 Dec;226:57-79. [Content Brief]

[3]. Cua DJ, et al. Innate IL-17-producing cells: the sentinels of the immune system. Nat Rev Immunol. 2010 Jul;10(7):479-89. [Content Brief]

[4]. Wright JF, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008 Aug 15;181(4):2799-805. [Content Brief]

[5]. Kinoshita H, et al. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol. 2009 Jan;123(1):179-86. [Content Brief]

[6]. Henness S, et al. IL-17A acts via p38 MAPK to increase stability of TNF-alpha-induced IL-8 mRNA in human ASM. Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1283-90. [Content Brief]

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Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells.The ED50 for this effect is 6.256 ng/mL, corresponding to a specific activity is 1.59×105 U/mg.

  • 1/16

    IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    Customer Validation
     HACAT cells were treated with different concentrations of IL-17A (0, 25, 50, 100 ng/mL) for 24 h. Lysates were immunoblotted with indicated antibodies. Sample numbers, n = 3. The results showed that IL-17A protein induced δ-catenin expression in a concentration-dependent manner.

  • 1/16

    IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    Customer Validation
     HACATs that silenced δ-ctn were then treated with 100 ng/mL of IL-17A in 24 h. Cells were performed with CCK8 assay, and the cell proliferation rate was measured by the absorbance at OD 450 nm.

  • 1/16

    IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    Customer Validation
     Images of EDU staining by HACATs that silenced δ-ctn were then treated with 100 ng/mL of IL-17A in 24 h, and cells were co-stained with DAPI to visualize nuclei. Bar = 50 μm. Sample numbers, n = 3.

  • 1/16

    IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: Clin Immunol. 2025 Aug:277:110494. [Abstract]

    Customer Validation
     IL-17A protein (100 ng/mL; 15 min-24 h) significantly downregulated the expression level of IκBα protein in KRT1-knockdown HaCaT cells.

  • 1/16

    IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: Clin Immunol. 2025 Aug:277:110494. [Abstract]

    Customer Validation
     RT-qPCR analysis of IL-8 expression in KRT1 knockdown cells stimulated with IL-17A (100 ng/mL) at 3 h, 6 h, 24 h. The results showed that in KRT1-knockdown cells, the expression level of IL-8 protein was significantly upregulated after stimulation with IL-17A protein for 3 h, 6 h, and 24 h, respectively.

  • IL-17A Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • Experimental Validation Results for IL-17A Protein, Human
  • IL-17A Protein, Human Product Image

  • IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    HACAT cells were treated with different concentrations of IL-17A (0, 25, 50, 100 ng/mL) for 24 h. Lysates were immunoblotted with indicated antibodies. Sample numbers, n = 3. The results showed that IL-17A protein induced δ-catenin expression in a concentration-dependent manner.

  • IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    HACATs that silenced δ-ctn were then treated with 100 ng/mL of IL-17A in 24 h. Cells were performed with CCK8 assay, and the cell proliferation rate was measured by the absorbance at OD 450 nm.

  • IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Apr 15;39(7):e70539. [Abstract]

    Images of EDU staining by HACATs that silenced δ-ctn were then treated with 100 ng/mL of IL-17A in 24 h, and cells were co-stained with DAPI to visualize nuclei. Bar = 50 μm. Sample numbers, n = 3.

  • IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: Clin Immunol. 2025 Aug:277:110494. [Abstract]

    IL-17A protein (100 ng/mL; 15 min-24 h) significantly downregulated the expression level of IκBα protein in KRT1-knockdown HaCaT cells.

  • IL-17A Protein, Human purchased from MedChemExpress. Usage Cited in: Clin Immunol. 2025 Aug:277:110494. [Abstract]

    RT-qPCR analysis of IL-8 expression in KRT1 knockdown cells stimulated with IL-17A (100 ng/mL) at 3 h, 6 h, 24 h. The results showed that in KRT1-knockdown cells, the expression level of IL-8 protein was significantly upregulated after stimulation with IL-17A protein for 3 h, 6 h, and 24 h, respectively.