TNF-α (31-45), human
Based on 13 publication(s) in Google Scholar
TNF-α (31-45), human is a potent NF-kB pathway activator. TNF-αis a proinflammatory cytokine that induces necrosis or apoptosis. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 144796-71-4
- Formula: C69H122N26O22
- Molecular Weight:1667.87
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications Citing Use of MedChemExpress (MCE) TNF-α (31-45), human
More- Chin Med. 2026 Mar 4;21(1):75. [Abstract]
- Int Immunopharmacol. 2026 Jan 1;168(Pt 1):115856. [Abstract]
- Eur J Pharmacol. 2023 Dec 15:961:176193. [Abstract]
- J Cell Commun Signal. 2025 Sep 21;19(3):e70045. [Abstract]
- Biomedicines. 2023 Dec 15;11(12):3314. [Abstract]
- Mol Med Rep. 2019 Oct;20(4):3565-3572. [Abstract]
- Chem Biol Drug Des. 2026 Apr;107(4):e70300. [Abstract]
- Front Oncol. 2022 Jun 30;12:804439. [Abstract]
- PLoS One. 2025 Apr 16;20(4):e0318311. [Abstract]
- Gene. 2026 Sep 20:1005:150233. [Abstract]
- Cytotechnology. 2021 Oct;73(5):727-743. [Abstract]
- Biocell. 2025 Apr 30.
- Authorea. 2025 Feb 19.
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WB
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Bio/Physico-chemical Assay
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WB
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WB
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RT-PCR
Biological Activity
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NF-κB |
TNF-α (31-45), human (5 μM; for 1 h) promotes cisplatin resistance by the activation of the NF-kB pathway. TNF-α (31-45), human does not alter RPS3 expression levels[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 144796-71-4
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Appearance Solid
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Molecular Weight 1667.87
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Formula C69H122N26O22
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Color White to off-white
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Sequence
Arg-Arg-Ala-Asn-Ala-Leu-Leu-Ala-Asn-Gly-Val-Glu-Leu-Arg-Asp
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Sequence Shortening
RRANALLANGVELRD
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (13)
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Journal Impact Factor
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Most Recent
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Chin Med
Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway. [Abstract]2026 Mar 4;21(1):75. PMID: 41776668 -
Int Immunopharmacol
Protective mechanism of zinc ions in spinal cord injury treatment: regulation of mitochondrial function and reduction of apoptosis via TNF-α pathway inhibition. [Abstract]2026 Jan 1;168(Pt 1):115856. PMID: 41253046 -
Eur J Pharmacol
Glycyrrhetinic acid attenuates endoplasmic reticulum stress-induced hepatocyte apoptosis via CHOP/DR5/Caspase 8 pathway in cholestasis. [Abstract]2023 Dec 15:961:176193. PMID: 37981257
TNF-α (31-45), human purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Dec 15:961:176193. [Abstract]
TNF-α(31-45), human (10 ng/mL; 24 h). The LDH level of supernatant from TNF-α-stimulated hepatocytes.
TNF-α (31-45), human purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Dec 15:961:176193. [Abstract]
TNF-α (31-45), human (10 ng/mL; 24 h). Immunoblotting of cleaved caspases 8 and 3 in TNF-α-stimulated hepatocytes. Blots were normalized to endogenous β-actin.
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J Cell Commun Signal
Integrated genomics reveals cellular senescence-driven molecular networks and immune crosstalk in myopia pathogenesis. [Abstract]2025 Sep 21;19(3):e70045. PMID: 40989170 -
Biomedicines
Thioacetamide-Induced Acute Liver Injury Increases Metformin Plasma Exposure by Downregulating Renal OCT2 and MATE1 Expression and Function. [Abstract]2023 Dec 15;11(12):3314. PMID: 38137535
TNF-α (31-45), human purchased from MedChemExpress. Usage Cited in: Biomedicines. 2023 Dec 15;11(12):3314. [Abstract]
TNF-α (31-45), human (0.5-2 μg/L; 48 h). Effects of dose-elevated TNF-α on the protein levels of MATE1 and OCT2 in HK-2 cells.
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Mol Med Rep
Expression and function of Toll‑like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis. [Abstract]2019 Oct;20(4):3565-3572. PMID: 31485664 -
Chem Biol Drug Des
Preliminary Study on the Mechanism of Arctigenin in Alleviating Osteoarthritis Pain by Suppressing Chondrocyte Pyroptosis: The Potential Role of the POU2F1/GRB10 Axis. [Abstract]2026 Apr;107(4):e70300. PMID: 42041039 -
Front Oncol
2022 Jun 30;12:804439. PMID: 35847905 -
PLoS One
Taurine is a potential therapy for rheumatoid arthritis via targeting FOXO3 through cellular senescence and autophagy. [Abstract]2025 Apr 16;20(4):e0318311. PMID: 40238799
TNF-α (31-45), human purchased from MedChemExpress. Usage Cited in: PLoS One. 2025 Apr 16;20(4):e0318311. [Abstract]
TNF-α (31-45), human (100 ng/mL; 24 h). In vitro experimental validation was performed using Western blot.
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Gene
Hirudin suppresses ovarian cancer cell proliferation and glycolysis through inhibiting the NF-κB/HK3 axis. [Abstract]2026 Sep 20:1005:150233. PMID: 42177920 -
Cytotechnology
miR-200b-3p alleviates TNF-α-induced apoptosis and inflammation of intestinal epithelial cells and ulcerative colitis progression in rats via negatively regulating KHDRBS1. [Abstract]2021 Oct;73(5):727-743. PMID: 34629748
TNF-α (31-45), human purchased from MedChemExpress. Usage Cited in: Cytotechnology. 2021 Oct;73(5):727-743. [Abstract]
TNF-α (31-45), human (0.5 ng/mL). The expression of miR-200b-3p in each group was detected by qRT-PCR.
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Solvent & Solubility
H2O : 50 mg/mL (29.98 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Idriss HT, Naismith JH. TNF alpha and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech. 2000 Aug 1;50(3):184-95. [Content Brief]
[2]. Xi Rui, et al. RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma. Front Oncol. 2022 Jun 30:12:804439. [Content Brief]
[3]. Jang DI, et al. The Role of Tumor Necrosis Factor Alpha (TNF-α) in Autoimmune Disease and Current TNF-α Inhibitors in Therapeutics. Int J Mol Sci. 2021 Mar 8;22(5):2719. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5996 mL | 2.9978 mL | 5.9957 mL | 14.9892 mL |
| 5 mM | 0.1199 mL | 0.5996 mL | 1.1991 mL | 2.9978 mL | |
| 10 mM | 0.0600 mL | 0.2998 mL | 0.5996 mL | 1.4989 mL | |
| 15 mM | 0.0400 mL | 0.1999 mL | 0.3997 mL | 0.9993 mL | |
| 20 mM | 0.0300 mL | 0.1499 mL | 0.2998 mL | 0.7495 mL | |
| 25 mM | 0.0240 mL | 0.1199 mL | 0.2398 mL | 0.5996 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.