1. Recombinant Proteins
  2. Immune Checkpoint Proteins
  3. Stimulatory immune checkpoint molecules
  4. CD40 & CD40L
  5. CD40L/CD154/TRAP Protein, Human

CD40L/CD154/TRAP Protein, Human

Cat. No.: HY-P7144
Handling Instructions

CD40L/CD154/TRAP Protein, Human exerts an important role in immune system regulation, including myeloid progenitor cell development.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $90 In-stock
Estimated Time of Arrival: December 31
50 μg $190 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

CD40L/CD154/TRAP Protein, Human Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P29965 (M113-L261)

Gene ID

959

Synonyms
rHuCD40L; CD154; TRAP; TNFSF5; CD40LG; CD40-L
AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Molecular Weight

Approximately 16.3 kDa

Biological Activity
The ED50 is <10 ng/mL as measured by PMB (Peripheral Mononuclear) cells, corresponding to a specific activity of >1.0 × 105 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 250 mM NaCl buffer.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

CD40, a member of the TNF receptor family of proteins, is a cell surface protein expressed on B lymphocytes, follicular dendritic cells, normal epithelium, and some epithelial carcinomas. Cells transfected with hCD40-L causes the proliferation of human tonsil B cells in the absence of costimuli and, in the presence of interleukin 4, induces immunoglobulin E secretion from purified human B cells[1]. The CD40 ligand interaction is initially described to play an essential role during membrane-membrane interactions between activated CD4+ T lymphocytes and B cells, as well as between CD4+ T cells and antigen-presenting cells. However, further studies show that soluble CD40 ligand (sCD40L) produced by T cells and platelets[2].

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CD40L/CD154/TRAP Protein, Human
Cat. No.:
HY-P7144
Quantity:
MCE Japan Authorized Agent: