MYL9 Protein, Human (P. pastoris, His)

MYL9 Protein, Human (P. pastoris, His) is the recombinant human-derived MYL9 protein, expressed by P. pastoris, with C-10*His tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: P. pastoris
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

MYL9 Protein, Human (P. pastoris, His) is the recombinant human-derived MYL9 protein, expressed by P. pastoris, with C-10*His tag.

Background

MYL9 is a myosin regulatory subunit that plays a crucial role in regulating contractile activity in both smooth muscle and nonmuscle cells via phosphorylation. It is involved in cytokinesis, receptor capping, and cell locomotion. In myoblasts, it may regulate PIEZO1-dependent cortical actomyosin assembly during myotube formation (By similarity).

Technical Parameters

  • Species Human
  • Source P. pastoris
  • Tag C-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • (S2-D172)
      Accession # P24844
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    MYL9; Myosin Regulatory Light Chain MRLC1; Myosin Light Chain 9; Myosin, Light Chain 9, Regulatory; MYRL2; Myosin Regulatory Light Chain 1; MRLC1; Myosin Regulatory Light Chain 9; MLC2; 20 KDa Myosin Light Chain; LC20; Myosin RLC; Myosin Regulatory Light

  • AA Sequence

    SSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

  • Predicted Molecular Mass

    21.7 kDa

  • Molecular Weight

    Approximately 18-22 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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