NSD3 Protein, Human (GST)
NSD3 Protein, Human (GST) is the recombinant human-derived NSD3, expressed by E. coli , with N-GST labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
NSD3 Protein, Human (GST) is the recombinant human-derived NSD3, expressed by E. coli , with N-GST labeled tag. ,
NSD3 is a histone methyltransferase that preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3, forming H3K4me2 and H3K27me2 modifications. H3 'Lys-4' methylation serves as a specific tag for epigenetic transcriptional activation, while 'Lys-27' methylation is associated with transcriptional repression.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-GST
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Accession
Q9BZ95-1 (E1021-D1322)
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Gene ID54904
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Molecular Construction
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N-term
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GST
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NSD3 (E1021-D1322)
Accession # Q9BZ95-1 -
C-term
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Protein Length
Partial
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Synonyms
NSD3; Protein Whistle; Prev. WHSC1L1; WHSC1-Like 1 Isoform 9 With Methyltransferase Activity To Lysine; WHISTLE; Wolf-Hirschhorn Syndrome Candidate 1-Like Protein 1; KMT3F; WHSC1-Like Protein 1; Wolf-Hirschhorn Syndrome Candidate 1-Like 1; Pp14328; Histon
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AA Sequence
EGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHED
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Predicted Molecular Mass
61 kDa
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Molecular Weight
Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)