NSD3 Protein, Human (GST)

NSD3 Protein, Human (GST) is the recombinant human-derived NSD3, expressed by E. coli , with N-GST labeled tag. ,

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

NSD3 Protein, Human (GST) is the recombinant human-derived NSD3, expressed by E. coli , with N-GST labeled tag. ,

Background

NSD3 is a histone methyltransferase that preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3, forming H3K4me2 and H3K27me2 modifications. H3 'Lys-4' methylation serves as a specific tag for epigenetic transcriptional activation, while 'Lys-27' methylation is associated with transcriptional repression.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-GST
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • GST
    • NSD3 (E1021-D1322)
      Accession # Q9BZ95-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NSD3; Protein Whistle; Prev. WHSC1L1; WHSC1-Like 1 Isoform 9 With Methyltransferase Activity To Lysine; WHISTLE; Wolf-Hirschhorn Syndrome Candidate 1-Like Protein 1; KMT3F; WHSC1-Like Protein 1; Wolf-Hirschhorn Syndrome Candidate 1-Like 1; Pp14328; Histon

  • AA Sequence

    EGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHED

  • Predicted Molecular Mass

    61 kDa

  • Molecular Weight

    Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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