PDGF R beta Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
PDGF Rβ protein is integral in embryonic development and regulates cell proliferation, survival, differentiation, chemotaxis, migration, and vascular development. It crucially recruits pericytes and smooth muscle cells to the endothelium, promoting vascular smooth muscle cell migration and neointimal formation at the site of injury. PDGF R beta Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PDGF R beta protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PDGF Rβ protein is integral in embryonic development and regulates cell proliferation, survival, differentiation, chemotaxis, migration, and vascular development. It crucially recruits pericytes and smooth muscle cells to the endothelium, promoting vascular smooth muscle cell migration and neointimal formation at the site of injury. PDGF R beta Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PDGF R beta protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
The receptor described in the text is the platelet-derived growth factor receptor alpha (PDGFRA). PDGFRA is involved in various cellular processes, including embryonic development, cell proliferation, survival, differentiation, chemotaxis, migration, and blood vessel development. It plays a crucial role in the recruitment of pericytes and smooth muscle cells to endothelial cells, as well as in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. PDGFRA is required for normal development of the cardiovascular system and for the formation of a branched network of capillaries in kidney glomeruli. It promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its ligands, including homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFD, leads to the activation of several signaling cascades. PDGFRA phosphorylates various downstream effectors, including PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1, NCK1, PDCD6IP/ALIX, and STAM. The receptor signaling is regulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, as well as by the rapid internalization of the activated receptor.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC50 of ≤5 ng/mL determined by ELISA.
3.Immobilized Biotinylated Human PDGF R beta, His-Avi Tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 6.0 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
P09619-1 (L33-F530)
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Molecular Construction
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N-term
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PDGF R beta (L33-F530)
Accession # P09619-1 -
8*His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
PDGFRB; PDGFR-Beta; Prev. PDGFR; Beta Platelet-Derived Growth Factor Receptor; PDGFR1; Activated Tyrosine Kinase PDGFRB; CD140b; CD140b Antigen; JTK12; NDEL1-PDGFRB; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; IBGC4; Beta-Type Platelet-Deri
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AA Sequence
LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPF
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Predicted Molecular Mass
59 kDa
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Molecular Weight
Approximately 78-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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