Semaphorin-6A/SEMA6A Protein, Human (HEK293, His)

Semaphorin-6A (SEMA6A) is the cell surface receptor for PLXNA2 and plays a key role in intercellular signaling. Its importance extends to the coordination of normal granule cell migration within the developing cerebellum and the promotion of actin cytoskeletal reorganization, which is critical for axonal guidance in the developing central nervous system. Semaphorin-6A/SEMA6A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-6A/SEMA6A protein, expressed by HEK293 , with C-His labeled tag.

Semaphorin-6A (SEMA6A) is a cell surface receptor for PLXNA2, crucial in cell-cell signaling and with significant involvement in various developmental processes. It is essential for normal granule cell migration in the developing cerebellum and plays a pivotal role in axon guidance within the central nervous system by promoting actin cytoskeleton reorganization. Functioning as a repulsive axon guidance cue, SEMA6A exhibits a repulsive action toward migrating granular neurons. Additionally, it may contribute to the channeling of sympathetic axons into sympathetic chains and regulate the temporal sequence of sympathetic target innervation. In the context of microbial infection, SEMA6A acts as a receptor for P. sordellii toxin TcsL in the vascular endothelium. This multifaceted protein thus plays a critical role in orchestrating cellular movements and axonal guidance during development and in response to microbial challenges.

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 6.389 μg/mL, corresponding to a specific activity is 1.565×10² U/mg.

Human

HEK293

C-His

Q9H2E6-1 (G19-T649)

57556  [NCBI]

Extracellular Domain

GFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNGTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDALFVCGTNAFNPSCRNYKMDTLEPFGDEFSGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGESPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIASVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLERYATSNEFPDDTLNFIKTHPLMDEAVPSIFNRPWFLRTMVRYRLTKIAVDTAAGPYQNHTVVFLGSEKGIILKFLARIGNSGFLNDSLFLEEMSVYNSEKCSYDGVEDKRIMGMQLDRASSSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWIKEGGACSHLSPNSRLTFEQDIERGNTDGLGDCHNSFVALNGHSSSLLPSTTTSDSTAQEGYESRGGMLDWKHLLDSPDSTDPLGAVSSHNHQDKKGVIRESYLKGHDQLVPVT

Approximately 85-110 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.