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Semaphorin-6A/SEMA6A Protein, Human (HEK293, His)

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Semaphorin-6A (SEMA6A) is the cell surface receptor for PLXNA2 and plays a key role in intercellular signaling. Its importance extends to the coordination of normal granule cell migration within the developing cerebellum and the promotion of actin cytoskeletal reorganization, which is critical for axonal guidance in the developing central nervous system. Semaphorin-6A/SEMA6A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-6A/SEMA6A protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Semaphorin-6A/SEMA6A Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Semaphorin-6A (SEMA6A) is the cell surface receptor for PLXNA2 and plays a key role in intercellular signaling. Its importance extends to the coordination of normal granule cell migration within the developing cerebellum and the promotion of actin cytoskeletal reorganization, which is critical for axonal guidance in the developing central nervous system. Semaphorin-6A/SEMA6A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-6A/SEMA6A protein, expressed by HEK293 , with C-His labeled tag.

Background

Semaphorin-6A (SEMA6A) is a cell surface receptor for PLXNA2, crucial in cell-cell signaling and with significant involvement in various developmental processes. It is essential for normal granule cell migration in the developing cerebellum and plays a pivotal role in axon guidance within the central nervous system by promoting actin cytoskeleton reorganization. Functioning as a repulsive axon guidance cue, SEMA6A exhibits a repulsive action toward migrating granular neurons. Additionally, it may contribute to the channeling of sympathetic axons into sympathetic chains and regulate the temporal sequence of sympathetic target innervation. In the context of microbial infection, SEMA6A acts as a receptor for P. sordellii toxin TcsL in the vascular endothelium. This multifaceted protein thus plays a critical role in orchestrating cellular movements and axonal guidance during development and in response to microbial challenges.

Verified Bioactivity

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 6.389 μg/mL, corresponding to a specific activity is 1.565×102 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9H2E6-1 (G19-T649)

  • Gene ID
    57556  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA6A (G19-T649)
      Accession # Q9H2E6-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SEMA6A; Sema Domain, Transmembrane Domain (TM), And Cytoplasmic Domain, (Semaphorin) 6A; Prev. SEMAQ; Sema VIa; Semaphorin VIA; SEMA6A-1; Semaphorin-6A; CDNA FLJ39018 Fis, Clone NT2RP7002594, Highly Similar To Semaphorin-6A; KIAA1368; Semaphorin 6A-1; SEM

  • AA Sequence

    GFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNGTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDALFVCGTNAFNPSCRNYKMDTLEPFGDEFSGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGESPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIASVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLERYATSNEFPDDTLNFIKTHPLMDEAVPSIFNRPWFLRTMVRYRLTKIAVDTAAGPYQNHTVVFLGSEKGIILKFLARIGNSGFLNDSLFLEEMSVYNSEKCSYDGVEDKRIMGMQLDRASSSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWIKEGGACSHLSPNSRLTFEQDIERGNTDGLGDCHNSFVALNGHSSSLLPSTTTSDSTAQEGYESRGGMLDWKHLLDSPDSTDPLGAVSSHNHQDKKGVIRESYLKGHDQLVPVT

  • Molecular Weight

    Approximately 85-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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