Serpin E2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Serpin E2 is a serine protease inhibitor that inhibits thrombin, trypsin, and urokinase. Its unique role in promoting neurite extension involves specific inhibition of thrombin activity. Serpin E2 Protein, Human (HEK293, His) is the recombinant human-derived Serpin E2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Serpin E2 is a serine protease inhibitor that inhibits thrombin, trypsin, and urokinase. Its unique role in promoting neurite extension involves specific inhibition of thrombin activity. Serpin E2 Protein, Human (HEK293, His) is the recombinant human-derived Serpin E2 protein, expressed by HEK293 , with C-6*His labeled tag.
Serpin E2, a serine protease inhibitor, exhibits inhibitory activity towards thrombin, trypsin, and urokinase. Notably, it plays a role in promoting neurite extension by specifically inhibiting thrombin activity. Through its interactions with thrombin, serpin E2 contributes to the regulation of proteolytic processes involved in various cellular functions. Additionally, the protein demonstrates binding affinity for heparin, suggesting its involvement in interactions with glycosaminoglycans and potentially modulating its activity in the context of cellular signaling or other biological processes.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 at room temperature for 30 minutes. The IC50 value is 0.306 nM.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P07093-2 (S20-P397)
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Molecular Construction
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N-term
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Serpin E2 (S20-P397)
Accession # P07093-2 -
6*His
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C-term
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Protein Length
Full Length of Isoform-2 Mature Protein
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Synonyms
SERPINE2; Serpin E2; Prev. PI7; PI-7; PN1; PN-1; GDN; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 2; PNI; Glial-Derived Neurite Promoting Factor; Serpin Peptidase Inhibitor, Clade E (Nexin, Pl
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AA Sequence
SHFNPLSLEELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITRSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINKP
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 10% glycerol, 8% sucrose, 0.05% Tween 80, pH 7.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM NaAc, 150 mM NaCl, pH 5.0, 10% glycerol.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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