SMPD2 Protein, Human (Cell-Free, His)
In vitro experiments have demonstrated that the sphingomyelin phosphodiesterase 2 (SMPD2) protein plays a key role in catalyzing the hydrolysis of sphingomyelin, leading to the formation of ceramide and phosphocholine. Furthermore, SMPD2 exhibits its enzymatic activity by hydrolyzing 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (hemolytic platelet-activating factor) in vivo. SMPD2 Protein, Human (Cell-Free, His) is the recombinant human-derived SMPD2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
In vitro experiments have demonstrated that the sphingomyelin phosphodiesterase 2 (SMPD2) protein plays a key role in catalyzing the hydrolysis of sphingomyelin, leading to the formation of ceramide and phosphocholine. Furthermore, SMPD2 exhibits its enzymatic activity by hydrolyzing 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (hemolytic platelet-activating factor) in vivo. SMPD2 Protein, Human (Cell-Free, His) is the recombinant human-derived SMPD2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Sphingomyelin Phosphodiesterase 2 (SMPD2) is an enzyme that catalyzes the hydrolysis of sphingomyelin, cleaving it into ceramide and phosphocholine. This enzymatic activity is essential for sphingolipid metabolism and the regulation of cellular signaling. SMPD2 is also involved in the hydrolysis of 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine, specifically lyso-platelet-activating factor, in vivo. Additionally, SMPD2 acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine. The diverse substrate specificity of SMPD2 highlights its role in modulating the levels of bioactive lipid molecules, including ceramides and lysophospholipids, which are implicated in various cellular processes, including cell proliferation, apoptosis, and inflammation. Understanding the functions of SMPD2 provides insights into the intricate regulation of lipid metabolism and its impact on cellular homeostasis.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
O60906 (M1-Q423)
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Gene ID6610
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Molecular Construction
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N-term
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10*His
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SMPD2 (M1-Q423)
Accession # O60906 -
C-term
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Protein Length
Full Length
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Synonyms
SMPD2; Lyso-PAF-PLC; Sphingomyelin Phosphodiesterase 2; N-SMase; NSMase; Sphingomyelin Phosphodiesterase 2, Neutral Membrane (Neutral Sphingomyelinase); Neutral Sphingomyelinase; NSMASE1; ISC1; NSMase1; Lyso-Platelet-Activating Factor-Phospholipase C
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AA Sequence
MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHRGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGAGEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRTKEQ
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Predicted Molecular Mass
49.1 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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