VEGF165 Protein, Human (Biotinylated, HEK293, Avi)

VEGF165 is a member of the PDGF/VEGF family encoding a heparin-binding homodimer that is crucial in inducing vascular endothelial cell proliferation and migration during angiogenesis. The VEGF gene is critical for blood vessel development, and its disruption leads to abnormal blood vessel formation in mouse embryos. VEGF165 Protein, Human (Biotinylated, HEK293, Avi) is the recombinant human-derived VEGF165 protein, expressed by HEK293 , with N-Avi labeled tag. The total length of VEGF165 Protein, Human (Biotinylated, HEK293, Avi) is 165 a.a., with molecular weight of 25-30 kDa.

VEGF165, a member of the PDGF/VEGF growth factor family, encodes a heparin-binding protein that forms a disulfide-linked homodimer. This growth factor plays a pivotal role in inducing proliferation and migration of vascular endothelial cells, crucial for both physiological and pathological angiogenesis. Disruption of the VEGF gene in mice resulted in aberrant embryonic blood vessel formation, underscoring its importance in vascular development. Elevated expression of VEGF is observed in various tumors, correlating with tumor stage and progression. Additionally, increased levels of VEGF are associated with conditions such as POEMS syndrome, microvascular complications of diabetes 1 (MVCD1), and atherosclerosis. Alternative splicing gives rise to different isoforms, including a C-terminally extended isoform with antiangiogenic properties. During infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), VEGF levels are heightened, contributing to inflammation by recruiting inflammatory cells and increasing the levels of angiopoietin II (Ang II), thereby establishing a feedback loop with Ang II to perpetuate the release of inflammatory cytokines. The broad expression of VEGF in tissues like the thyroid, prostate, and 21 other tissues emphasizes its widespread involvement in diverse physiological processes.

Measured by its binding ability in a functional ELISA. Immobilized Anti-VEGFA antibody at 5 μg/mL can bind Biotinylated Human VEGF165.The EC₅₀ is 6.080-7.120 ng/mL.

Human

HEK293

N-Avi

NP_001165097 (A27-R191)

7422  [NCBI]

25-30 kDa

Greater than 95% as determined by reduced SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.