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Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
114 Results for "
Real-time
" in MedChemExpress (MCE) Product Catalog:
Products (114)
114 Results for "Real-time" in MCE Product Catalog:
Cat. No.: HY-D2939
CAS No.: 1622078-12-9
Cat. No.: HY-W1061847
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5-VIC Phosphoramidite is a Fluorescent dye and a component of TaqMan probes for nucleic acid detection. 5-VIC Phosphoramidite serves as a 5' reporter dye, which is conjugated to probes targeting the conserved region of the S protein of porcine epidemic diarrhea virus genotype GII, and is used with Eclipse® Dark Quencher for duplex real-time quantitative PCR. 5-VIC Phosphoramidite-labeled probes targeting the Rv0222 gene in the RD4 region specific to Mycobacterium tuberculosis are applied in triplex droplet digital PCR to differentiate mycobacterial strains. During PCR amplification, the 5'→3' exonuclease activity of Taq polymerase mediates the separation of 5-VIC Phosphoramidite from the quencher, thereby generating a detectable fluorescent signal .
Cat. No.: HY-D3224
CAS No.: 2413777-91-8
Cat. No.: HY-D3110
CAS No.: 2757682-03-2
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NRGolgi is a Nile Red-based solvatochromic fluorescent probe targeted to the Golgi apparatus. NRGolgi localizes selectively to the Golgi membrane via its myristic acid group, and leverages the property of its emission wavelength undergoing blue/red shift with local polarity to quantitatively detect the lipid order of the Golgi membrane in living cells using dual-emission ratiometric imaging (550-600 nm / 600-650 nm). NRGolgi is suitable for real-time monitoring of Golgi lipid order under conditions of oxidative stress, mechanical stress, and altered cholesterol metabolism .
Cat. No.: HY-D3269
CAS No.: 2088127-60-8
Cat. No.: HY-D3398
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FlavAc is a fluorescent dye (with an excitation wavelength of 980 nm) for real-time in vivo imaging of lipid metabolism disorders, including early fatty liver disease and atherosclerotic plaques. FlavAc specifically integrates into endogenous lipoproteins, primarily low-density lipoprotein (LDL), and shows no brightness enhancement when bound to albumin. FlavAc forms aggregates in aqueous solutions, leading to fluorescence quenching; however, when integrated into lipoproteins, such aggregation decreases, forming stable fluorescent complexes with enhanced brightness. FlavAc can selectively illuminate LDL upon laser irradiation .
Cat. No.: HY-180983
Azo-PROTAC-4C-trans is a BCR-ABL PROTAC degrader that can efficiently degrade the BCR-ABL fusion protein and ABL protein. Azo-PROTAC-4C-trans exhibits potent selective anti-proliferative activity against K562 cells. Azo-PROTAC-4C-trans allows real-time, reversible regulation of its activity via UV (to inactivate it) /visible light (to activate it) irradiation. Azo-PROTAC-4C-trans can be used for the study of myeloid leukemia .
Cat. No.: HY-D3160
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Golgi-hNR is a cell membrane-permeable Golgi-targeted fluorescent probe containing a sulfonamide group. Golgi-hNR selectively accumulates in the Golgi apparatus of living cells, enabling real-time tracking and homeostasis assessment of reversible pH changes in the Golgi apparatus. Golgi-hNR exhibits a pH-dependent "off-on" fluorescence response mechanism within the pH 7.4-3.0 range, and its red fluorescence intensity decreases with increasing pH. The Ex/Em of Golgi-hNR is 560/664 nm and 580/664 nm, with the maximum emission peak located at 615 nm .
Cat. No.: HY-D3195
CAS No.: 2028316-19-8
Cat. No.: HY-D3364
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RL480 is a green-emitting RNA fluorescent dye used for selective RNA labeling and imaging. RL480 contains an acylimidazole reactive group that undergoes selective acylation with the 2′-OH group of RNA ribose, and restricts intramolecular rotation through covalent binding to relieve the TICT quenching effect, thereby generating a fluorescence enhancement signal. RL480 enables selective fluorescent labeling of RNA over DNA, and is applicable to RNA gel detection, real-time RNA synthesis monitoring and live-cell RNA imaging. The detection wavelengths are Ex/Em = 480 nm/514 nm .
Cat. No.: HY-D3099
CAS No.: 3078752-74-3
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CSMPP is a ratiometric lysosomal pH fluorescent probe with aggregation-induced emission (AIE) properties, which is used for real-time quantitative detection of lysosomal pH changes in living cells and in vivo. CSMPP has a cyanodistyrene backbone, and contains N-methylpiperazine and pyridine groups (pH-responsive); protonation of the pyridine group significantly enhances intramolecular charge transfer (ICT), leading to green fluorescence emission (Ex/Em = 405/503 nm) under neutral/basic conditions and red fluorescence emission (Ex/Em = 405/615 nm) under acidic conditions. CSMPP exhibits high selectivity, reversibility, good biocompatibility and excellent photostability .
Cat. No.: HY-D3366
Target:
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Th-TMR-S-Halo is a fluorescent probe for HaloTag labeling and live-cell imaging, including super-resolution imaging and real-time visualization of endoplasmic reticulum (ER) vortex formation under ER stress conditions. Th-TMR-S-Halo shows weak fluorescence in the free state, and upon covalent binding to HaloTag, fluorescence activation is achieved by restricting the rotation of the thiophene ring. Th-TMR-S-Halo can be used in studies related to intracellular protein localization, cytoskeleton structure, and ER vortex formation. Live-cell NSPARC super-resolution detection is performed using Ex/Em = 561/570-616 nm .
Cat. No.: HY-D3436
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Pt-Luc is a bioluminescent probe used for real-time, non-invasive monitoring of Pt (IV) prodrug reduction in living cells and animal models. The detection mechanism of Pt-Luc relies on the intracellular reduction mediated by active cysteine residues in proteins. Pt-Luc can penetrate the membrane of living cells; after entering cells, active cysteine thiolates in high-molecular-weight protein components reduce the Pt (IV) complex, thereby cleaving the caged aminoluciferin substrate. The released free aminoluciferin is then oxidized by luciferase in the presence of ATP to generate a bioluminescent signal, whose intensity is directly correlated with the reduction degree of Pt (IV) .
Cat. No.: HY-D3087
CAS No.: 1966158-27-9
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Lyso-OC is a two-photon fluorescent probe that enables real-time monitoring of autophagy through ultrasensitive detection of lysosomal polarity changes. Lyso-OC possesses a coumarin (HY-N0709) solvatochromic group and a morpholine lysosome-targeting group, allowing selective lysosomal localization in living cells. Lyso-OC emits strong fluorescence in low-polarity lysosomes, whereas in high-polarity autolysosomes formed during autophagy, its fluorescence intensity decreases and the emission peak shifts by approximately 30 nm. For two-photon detection, the excitation/emission wavelengths of Lyso-OC are 760/490-550 nm, and its one-photon excitation wavelength is 375 nm. Lyso-OC can be used in cancer-related research .
Cat. No.: HY-D3146
CAS No.: 1418017-91-0
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Di-4-AN (F) EPPTEA is a voltage-sensitive dye used for membrane potential imaging and optical recording of electrical activity in excitable cells and tissues. Its detection mechanism is based on electrochromism (also known as the molecular Stark effect). The single-photon excitation/emission wavelengths measured in ethanol solution are Ex/Em = 444/610 nm, and it can be excited via 1064 nm two-photon excitation in optical recording applications. Di-4-AN (F) EPPTEA can be used to record backpropagating action potentials in individual dendritic spines of brain cortical slices, map subcellular action potential characteristics of single cardiomyocytes, and perform real-time recording of multicellular action potentials in cerebellar slices .
Cat. No.: HY-D3311
Cat. No.: HY-D3108
CAS No.: 2569032-08-0
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BV-1 is a fluorescent probe for viscosity detection and imaging in living cells. BV-1 can be used to detect changes in intracellular viscosity and mitochondrial viscosity in living cells. BV-1 responds to environmental viscosity via a molecular rotor mechanism. In a low-viscosity environment, the centrally located unsaturated structure induces a twisted intramolecular charge transfer (TICT) state and promotes non-radiative transitions, resulting in fluorescence quenching; when viscosity increases, molecular rotation is restricted, the TICT state is attenuated, and fluorescence is enhanced with a red shift. BV-1 can be used for real-time monitoring of changes in cytoplasmic viscosity and the viscosity increase during cell apoptosis. Ex/Em = 480/570 nm .
Cat. No.: HY-D3181
Cat. No.: HY-D3198
CAS No.: 2351937-34-1
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NIR-fluorescent ceramide is a neutral near-infrared (NIF) fluorescent probe (λem=650 nm) based on the C-bridged nitrobenzoxadiazole SCOTfluor core, which enables visualization of lipid metabolic processes such as sphingolipid trafficking. The emission signal of NIR-fluorescent ceramide increases significantly in liposomal environments. NIR-fluorescent ceramide acts as an intracellular trafficking tracer, localizing to regions surrounding the endoplasmic reticulum and Golgi apparatus within a short period, and then translocating to recycling lysosomes over a longer period, thus enabling real-time visualization of sphingolipid trafficking and biological lipid functions in living cells. NIR-fluorescent ceramide also generates metabolic uptake profiles and provides multiple metabolic readouts in human cancer cell lines .
Cat. No.: HY-D2991
CAS No.: 2081872-38-8
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Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
