CDr15 

CDr15 is a deep-red fluorescent probe (Ex=733 nm) that can selectively intercalate into and label bacterial extracellular DNA (eDNA). CDr15 exhibits extremely high specificity for biofilm imaging and cannot effectively bind to mammalian nuclear DNA. CDr15 enables real-time visualization of the microcolony structure and developmental process of three-dimensional *Pseudomonas aeruginosa* biofilms, and accurately localizes biofilm-forming regions of microorganisms in a mouse corneal infection model. With low background interference signals, CDr15 serves as an ideal diagnostic tool for research fields including bacterial biofilms and corneal infections^[1].

CDr15 binds to DNA via intercalation, with enhanced fluorescence upon binding, and exhibits differential fluorescence responses to DNA versus RNA^[1].
CDr15 (stained for 30 minutes; biofilm cultured for 1-5 days) selectively stains the eDNA component within the extracellular matrix of P. aeruginosa biofilms, with distinct spatial localization in developing biofilms, while not staining biofilms lacking eDNA or planktonic bacteria^[1].
CDr15 does not stain the nuclei of live or treated HEK293 cells and is selective for mammalian nuclear DNA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

472.34

C₂₇H₂₇BF₂N₄O

2028316-19-8

CC(C=C1/C=C/C2=CC=C(N(CCO)C)C=C2)=C(N1[B-]3(F)F)C(C4=CC=C(N)C=C4)=C5[N+]3=CC=C5

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kwon HY , et al. Visualizing biofilm by targeting eDNA with long wavelength probe CDr15. Biomater Sci. 2019;7(9):3594-3598.