CDr15
CDr15 is a deep-red fluorescent probe (Ex=733 nm) that can selectively intercalate into and label bacterial extracellular DNA (eDNA). CDr15 exhibits extremely high specificity for biofilm imaging and cannot effectively bind to mammalian nuclear DNA. CDr15 enables real-time visualization of the microcolony structure and developmental process of three-dimensional *Pseudomonas aeruginosa* biofilms, and accurately localizes biofilm-forming regions of microorganisms in a mouse corneal infection model. With low background interference signals, CDr15 serves as an ideal diagnostic tool for research fields including bacterial biofilms and corneal infections.
For research use only. We do not sell to patients.
- CAS No.: 2028316-19-8
- Formula: C27H27BF2N4O
- Molecular Weight:472.34
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
CDr15 binds to DNA via intercalation, with enhanced fluorescence upon binding, and exhibits differential fluorescence responses to DNA versus RNA[1].
CDr15 (stained for 30 minutes; biofilm cultured for 1-5 days) selectively stains the eDNA component within the extracellular matrix of P. aeruginosa biofilms, with distinct spatial localization in developing biofilms, while not staining biofilms lacking eDNA or planktonic bacteria[1].
CDr15 does not stain the nuclei of live or treated HEK293 cells and is selective for mammalian nuclear DNA[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2028316-19-8
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Molecular Weight 472.34
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Formula C27H27BF2N4O
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SMILES
CC(C=C1/C=C/C2=CC=C(N(CCO)C)C=C2)=C(N1[B-]3(F)F)C(C4=CC=C(N)C=C4)=C5[N+]3=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CDr15 Related Classifications
HY-D3195 Related Classifications
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