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Tetrazolium blue

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By Targets:	Biochemical Assay Reagents (1) COX (2) Endogenous Metabolite (1) Fluorescent Dye (3) Interleukin Related (2) NO Synthase (2) Reactive Oxygen Species (ROS) (2) Reference Standards (1)
By Research Areas:	Inflammation/Immunology (2) Metabolic Disease (1)

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Fluorescent Dyes

Biochemical Assay Reagents

Natural
Products

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-15924

Thiazolyl Blue 180+ Cited Publications MTT; Thiazolyl blue Tetrazolium bromide; Methylthiazolyldiphenyl-Tetrazolium bromide	Fluorescent Dye	Others
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .

HY-15925

*Nitro blue* tetrazolium chloride** 5+ Cited Publications NBT	Fluorescent Dye	Others
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures .

HY-107208

Procyanidol B4 1 Publications Verification (-)-Procyanidin B4	Reactive Oxygen Species (ROS) COX Interleukin Related NO Synthase	Inflammation/Immunology
Procyanidol B4 ((-)-Procyanidin B4) is an orally active polyphenol found in Q.gilva. Procyanidol B4 exerts antioxidant activity by scavenging DPPH (HY-112053) (IC₅₀: 12.15 μM) and Nitro blue tetrazolium chloride (NBT) (HY-15925) (IC₅₀: 8.67 μM). Procyanidol B4 exhibits anti-inflammatory activity by inhibiting the expression of COX-2 and IL-1β and iNOS .

HY-D0007

**Blue Tetrazolium** Tetrazolium blue	Biochemical Assay Reagents	Others
Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm .

HY-P2800A

Diaphorase, clostridium sp.	Endogenous Metabolite	Metabolic Disease
Diaphorase, Clostridium sp. is a diaphorase derived from Clostridium sp. As a flavin-containing NAD (P) H dehydrogenase, Diaphorase, Clostridium sp. uses NADH as an electron donor to reduce artificial dyes such as DCPIP and NBT, and participates in energy metabolism .

HY-DY1086

Thiazolyl Blue (solution) MTT (solution); Thiazolyl blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-Tetrazolium bromide (solution)	Fluorescent Dye	Others
Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis . Solvent and concentration: PBS: 5 mg/mL

HY-107208R

Procyanidol B4 (Standard) (-)-Procyanidin B4 (Standard)	Reference Standards Reactive Oxygen Species (ROS) COX Interleukin Related NO Synthase	Inflammation/Immunology
Procyanidol B4 (Standard) is the analytical standard of Procyanidol B4 (HY-107208). This product is intended for research and analytical applications. Procyanidol B4 ((-)-Procyanidin B4) is an orally active polyphenol found in Q.gilva. Procyanidol B4 exerts antioxidant activity by scavenging DPPH (HY-112053) (IC₅₀: 12.15 μM) and Nitro blue tetrazolium chloride (NBT) (HY-15925) (IC₅₀: 8.67 μM). Procyanidol B4 exhibits anti-inflammatory activity by inhibiting the expression of COX-2 and IL-1β and iNOS .

Cat. No.	Product Name	Type

HY-15924

Thiazolyl Blue 180+ Cited Publications MTT; Thiazolyl blue Tetrazolium bromide; Methylthiazolyldiphenyl-Tetrazolium bromide	Fluorescent Dyes
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .

HY-15925

*Nitro blue* tetrazolium chloride** 5+ Cited Publications NBT	Fluorescent Dyes
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures .

HY-DY1086

Thiazolyl Blue (solution) MTT (solution); Thiazolyl blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-Tetrazolium bromide (solution)	Fluorescent Dyes
Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis . Solvent and concentration: PBS: 5 mg/mL

Thiazolyl Blue (solution)

MTT (solution); Thiazolyl blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-Tetrazolium bromide (solution)

Fluorescent Dyes

Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .
Solvent and concentration: PBS: 5 mg/mL

Cat. No.	Product Name	Type

HY-D0007

**Blue Tetrazolium** Tetrazolium blue	Biochemical Assay Reagents
Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm .

Blue Tetrazolium

Tetrazolium blue

Biochemical Assay Reagents

Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm .

Procyanidol B4 1 Publications Verification (-)-Procyanidin B4	Structural Classification Flavonoids other families Classification of Application Fields Phenols Polyphenols Plants Biflavones Inflammation/Immunology Disease Research Fields Source Classification	Reactive Oxygen Species (ROS) COX Interleukin Related NO Synthase
Procyanidol B4 ((-)-Procyanidin B4) is an orally active polyphenol found in Q.gilva. Procyanidol B4 exerts antioxidant activity by scavenging DPPH (HY-112053) (IC₅₀: 12.15 μM) and Nitro blue tetrazolium chloride (NBT) (HY-15925) (IC₅₀: 8.67 μM). Procyanidol B4 exhibits anti-inflammatory activity by inhibiting the expression of COX-2 and IL-1β and iNOS .

Procyanidol B4 (Standard) (-)-Procyanidin B4 (Standard)	Structural Classification Flavonoids other families Phenols Polyphenols Plants Biflavones Source Classification	Reference Standards Reactive Oxygen Species (ROS) COX Interleukin Related NO Synthase
Procyanidol B4 (Standard) is the analytical standard of Procyanidol B4 (HY-107208). This product is intended for research and analytical applications. Procyanidol B4 ((-)-Procyanidin B4) is an orally active polyphenol found in Q.gilva. Procyanidol B4 exerts antioxidant activity by scavenging DPPH (HY-112053) (IC₅₀: 12.15 μM) and Nitro blue tetrazolium chloride (NBT) (HY-15925) (IC₅₀: 8.67 μM). Procyanidol B4 exhibits anti-inflammatory activity by inhibiting the expression of COX-2 and IL-1β and iNOS .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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Tetrazolium blue

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

NaturalProducts

Natural
Products