tert-Butyl N-(4-azidobutyl)carbamate
Based on 1 Customer Validation
tert-Butyl N-(4-azidobutyl)carbamate is N-Boc protected crosslinker. tert-Butyl N-(4-azidobutyl)carbamate can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The Boc group can be deprotected under mild acidic conditions to form the free amine.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 129392-85-4
- Formula: C9H18N4O2
- Molecular Weight:214.26
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
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Help & FAQs
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Biological Activity
Description
Chemical Information
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CAS No. 129392-85-4
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Appearance Liquid
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Molecular Weight 214.26
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Formula C9H18N4O2
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Color Colorless to light yellow
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SMILES
O=C(OC(C)(C)C)NCCCCN=[N+]=[N-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (466.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (239 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.6672 mL | 23.3361 mL | 46.6723 mL | 116.6807 mL |
| 5 mM | 0.9334 mL | 4.6672 mL | 9.3345 mL | 23.3361 mL | |
| 10 mM | 0.4667 mL | 2.3336 mL | 4.6672 mL | 11.6681 mL | |
| 15 mM | 0.3111 mL | 1.5557 mL | 3.1115 mL | 7.7787 mL | |
| 20 mM | 0.2334 mL | 1.1668 mL | 2.3336 mL | 5.8340 mL | |
| 25 mM | 0.1867 mL | 0.9334 mL | 1.8669 mL | 4.6672 mL | |
| 30 mM | 0.1556 mL | 0.7779 mL | 1.5557 mL | 3.8894 mL | |
| 40 mM | 0.1167 mL | 0.5834 mL | 1.1668 mL | 2.9170 mL | |
| 50 mM | 0.0933 mL | 0.4667 mL | 0.9334 mL | 2.3336 mL | |
| 60 mM | 0.0778 mL | 0.3889 mL | 0.7779 mL | 1.9447 mL | |
| 80 mM | 0.0583 mL | 0.2917 mL | 0.5834 mL | 1.4585 mL | |
| 100 mM | 0.0467 mL | 0.2334 mL | 0.4667 mL | 1.1668 mL |
tert-Butyl N-(4-azidobutyl)carbamate Related Classifications
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