HRO761
Based on 6 publication(s) in Google Scholar
HRO761 (Werner syndrome RecQ helicase-IN-1) (example 42) is a potent Werner syndrome RecQ DNA helicase enzyme (WRN) inhibitor and can be used in cancer research.
For research use only. We do not sell to patients.
- Purity : 99.52%
- CAS No.: 2869954-34-5
- Formula: C31H31ClF3N9O5
- Molecular Weight:702.08
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) HRO761
More- Cancer Res. 2026 Jul 16:10.1158/0008-5472.CAN-25-3567. [Abstract]
- Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(8):e2413368. [Abstract]
- Cell Rep. 2026 Jul 28;45(7):117686.
- Mol Cancer Ther. 2026 Jul 15:10.1158/1535-7163.MCT-26-0182.
- Sci Rep. 2024 Sep 6;14(1):20824. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
IC50 & Target
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Helicase |
In Vitro
HRO761 (Werner syndrome RecQ helicase-IN-1) (Example 42) has an IC50 value of 100 nM for WRN ATPase and GI50 values of 50 nM and >10 μM for SW48 and DLD1 WRN-KO cell lines, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2869954-34-5
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Appearance Solid
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Molecular Weight 702.08
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Formula C31H31ClF3N9O5
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Color White to light yellow
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SMILES
O=C(N1CCN(CC1)C2=C(CC)N(CC(NC3=CC=C(C(F)(F)F)C=C3Cl)=O)C4=NC(C5=CCOCC5)=NN4C2=O)C6=NC=NC(C)=C6O
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Synonyms
Werner syndrome RecQ helicase-IN-1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Cancer Res
Targeting WRN Helicase in Microsatellite Instable Colorectal Cancer Induces Antitumor Immunity through Extrachromosomal Circular DNA Release. [Abstract]2026 Jul 16:10.1158/0008-5472.CAN-25-3567. PMID: 42461083 -
Nat Commun
DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells. [Abstract]2025 Aug 25;16(1):7901. PMID: 40854910
HRO761 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]
Representative immunofluorescence images and quantification of prometaphase cells showed MiDAS. HRO761 (10 μM; 1.5 h) reduced MiDAS significantly in U2OS cells.
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Adv Sci (Weinh)
2025 Feb;12(8):e2413368. PMID: 39737862
HRO761 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(8):e2413368. [Abstract]
Imaging and relative fluorescence intensity of the ATED probe in HCT116 cells treated with HRO761 (HRO-761) (2–30 μM; 2 h). HRO761 (HRO-761) significantly decreased the intracellular fluorescence signal in a dose-dependent manner, reflecting a progressive decline in WRN enzymatic activity.
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Sci Rep
PROTAC-mediated conditional degradation of the WRN helicase as a potential strategy for selective killing of cancer cells with microsatellite instability. [Abstract]2024 Sep 6;14(1):20824. PMID: 39242638
HRO761 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Sep 6;14(1):20824. [Abstract]
Graph represented the ratio of mCherry HCT-116 Bd-WRN clone 44 to BFP SW620 Bd-WRN clone 17 at 0 h, 48 h and 96 h after treatment with 0.1% DMSO (UT) or HRO761 (40–400 nM) from four biological repeats.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (142.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4243 mL | 7.1217 mL | 14.2434 mL | 35.6085 mL |
| 5 mM | 0.2849 mL | 1.4243 mL | 2.8487 mL | 7.1217 mL | |
| 10 mM | 0.1424 mL | 0.7122 mL | 1.4243 mL | 3.5608 mL | |
| 15 mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 2.3739 mL | |
| 20 mM | 0.0712 mL | 0.3561 mL | 0.7122 mL | 1.7804 mL | |
| 25 mM | 0.0570 mL | 0.2849 mL | 0.5697 mL | 1.4243 mL | |
| 30 mM | 0.0475 mL | 0.2374 mL | 0.4748 mL | 1.1869 mL | |
| 40 mM | 0.0356 mL | 0.1780 mL | 0.3561 mL | 0.8902 mL | |
| 50 mM | 0.0285 mL | 0.1424 mL | 0.2849 mL | 0.7122 mL | |
| 60 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5935 mL | |
| 80 mM | 0.0178 mL | 0.0890 mL | 0.1780 mL | 0.4451 mL | |
| 100 mM | 0.0142 mL | 0.0712 mL | 0.1424 mL | 0.3561 mL |
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