Imidazoleacetic acid hydrochloride
Based on 2 publication(s) in Google Scholar
Imidazoleacetic acid hydrochloride is a γ-aminobutyric acid receptor agonist. Imidazoleacetic acid hydrochloride inhibits GABA-T in a non-competitive manner, with a Ki value of 0.34 mM. Imidazoleacetic acid hydrochloride increases total free GABA in brain.
For research use only. We do not sell to patients.
- Purity : 99.94%
- CAS No.: 3251-69-2
- Formula: C5H7ClN2O2
- Molecular Weight:162.58
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-W007686R
Imidazoleacetic acid hydrochloride (Standard) -
HY-W045271
Imidazole-5-propionic acid -
HY-W007321
1H-Imidazole-2-carbaldehyde -
HY-N0438
Pimpinellin -
HY-W007695
1H-Imidazole-4-carboxylic acid -
HY-41461A
5-Amino-3H-imidazole-4-carboxamide hydrochloride -
HY-W002802
4-Iodo-1H-imidazole -
HY-W016155
2-Butyl-4-chloro-5-formylimidazole -
HY-W016155R
2-Butyl-4-chloro-5-formylimidazole (Standard) -
HY-W045271R
Imidazole-5-propionic acid (Standard) -
HY-41461AR
5-Amino-3H-imidazole-4-carboxamide hydrochloride (Standard)
Publications Citing Use of MedChemExpress (MCE) Imidazoleacetic acid hydrochloride
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Microbial Metabolite |
Human Endogenous Metabolite |
In Vitro
Imidazoleacetic acid (3 mM) hydrochloride inhibits GABA-T in a non-competitive manner, with a Ki value of 0.34 mM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3251-69-2
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Appearance Solid
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Molecular Weight 162.58
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Formula C5H7ClN2O2
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Color Off-white to light yellow
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SMILES
O=C(O)CC1=CN=CN1.[H]Cl
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Synonyms
Imidazolyl-4-acetic acid hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
FASEB J
The metabolomic profiling identifies N, N-dimethylglycine as a facilitator of dorsal root ganglia neuron axon regeneration after injury. [Abstract]2022 May;36(5):e22305. PMID: 35394692
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (615.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (307.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.38 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.38 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (615.08 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Prell GD, et al. Imidazoleacetic acid-ribotide: an endogenous ligand that stimulates imidazol(in)e receptors. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13677-82. [Content Brief]
[2]. Clifford JM, et al. Biochemical and pharmacological actions of imidazoleacetic acid. Biochem Pharmacol. 1973 Feb 15;22(4):535-42. [Content Brief]
[3]. Thomas B, et al. Imidazoleacetic acid, a gamma-aminobutyric acid receptor agonist, can be formed in rat brain by oxidation of histamine. J Neurochem. 1995 Aug;65(2):818-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.1508 mL | 30.7541 mL | 61.5082 mL | 153.7704 mL |
| 5 mM | 1.2302 mL | 6.1508 mL | 12.3016 mL | 30.7541 mL | |
| 10 mM | 0.6151 mL | 3.0754 mL | 6.1508 mL | 15.3770 mL | |
| 15 mM | 0.4101 mL | 2.0503 mL | 4.1005 mL | 10.2514 mL | |
| 20 mM | 0.3075 mL | 1.5377 mL | 3.0754 mL | 7.6885 mL | |
| 25 mM | 0.2460 mL | 1.2302 mL | 2.4603 mL | 6.1508 mL | |
| 30 mM | 0.2050 mL | 1.0251 mL | 2.0503 mL | 5.1257 mL | |
| 40 mM | 0.1538 mL | 0.7689 mL | 1.5377 mL | 3.8443 mL | |
| 50 mM | 0.1230 mL | 0.6151 mL | 1.2302 mL | 3.0754 mL | |
| 60 mM | 0.1025 mL | 0.5126 mL | 1.0251 mL | 2.5628 mL | |
| 80 mM | 0.0769 mL | 0.3844 mL | 0.7689 mL | 1.9221 mL | |
| 100 mM | 0.0615 mL | 0.3075 mL | 0.6151 mL | 1.5377 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Imidazoleacetic acid hydrochloride Related Classifications
HY-W007686 Related Classifications
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