23,25-Dihydroxy-24-oxovitamin D3
Based on 1 Customer Validation
23,25-Dihydroxy-24-oxovitamin D3 is a major metabolite of 24(R),25-Dihydroxyvitamin D3. 23,25-Dihydroxy-24-oxovitamin D3 can be used for the research of metabolic diseases.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 84164-55-6
- Formula: C27H42O4
- Molecular Weight:430.62
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
23,25-Dihydroxy-24-oxovitamin D3 is a major metabolite of 24(R),25-Dihydroxyvitamin D3[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 84164-55-6
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Appearance Solid
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Molecular Weight 430.62
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Formula C27H42O4
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Color White to off-white
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SMILES
CC(C)(O)C(C(O)C[C@H]([C@H]1CC[C@@]2([H])/C(CCC[C@]12C)=C/C=C3C(CC[C@H](O)C\3)=C)C)=O
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Synonyms
24-Oxo-23,25-dihydroxyvitamin D3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (232.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3222 mL | 11.6112 mL | 23.2223 mL | 58.0558 mL |
| 5 mM | 0.4644 mL | 2.3222 mL | 4.6445 mL | 11.6112 mL | |
| 10 mM | 0.2322 mL | 1.1611 mL | 2.3222 mL | 5.8056 mL | |
| 15 mM | 0.1548 mL | 0.7741 mL | 1.5482 mL | 3.8704 mL | |
| 20 mM | 0.1161 mL | 0.5806 mL | 1.1611 mL | 2.9028 mL | |
| 25 mM | 0.0929 mL | 0.4644 mL | 0.9289 mL | 2.3222 mL | |
| 30 mM | 0.0774 mL | 0.3870 mL | 0.7741 mL | 1.9352 mL | |
| 40 mM | 0.0581 mL | 0.2903 mL | 0.5806 mL | 1.4514 mL | |
| 50 mM | 0.0464 mL | 0.2322 mL | 0.4644 mL | 1.1611 mL | |
| 60 mM | 0.0387 mL | 0.1935 mL | 0.3870 mL | 0.9676 mL | |
| 80 mM | 0.0290 mL | 0.1451 mL | 0.2903 mL | 0.7257 mL | |
| 100 mM | 0.0232 mL | 0.1161 mL | 0.2322 mL | 0.5806 mL |
23,25-Dihydroxy-24-oxovitamin D3 Related Classifications
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