6-JOE alkyne, CHC protected
6-JOE alkyne (CHC-protected) is a derivative of JOE (dimethoxy-dichloro-carboxyfluorescein) featuring a terminal alkyne group for use in click chemistry reactions (CuAAC); the CHC (cyclohexylcarbodiimide) protecting group ensures stability during oligonucleotide synthesis. 6-JOE alkyne (CHC-protected) is primarily used for click chemistry-based bioconjugation (Ex/Em = 520/548 nm).
For research use only. We do not sell to patients.
- Formula: C40H37Cl2NO10
- Molecular Weight:762.63
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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Molecular Weight 762.63
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Formula C40H37Cl2NO10
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SMILES
O=C(NCC#C)C1=CC=C2C(OC3(C2=C1)C4=C(C(Cl)=C(C(OC)=C4)OC(C5CCCCC5)=O)OC6=C3C=C(C(OC(C7CCCCC7)=O)=C6Cl)OC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
6-JOE alkyne, CHC protected Related Classifications
HY-D3507 Related Classifications
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