Cat. No.: HY-B0394

Atropine sulfate monohydrate

(Synonyms: Tropine tropate sulfate monohydrate; DL-Hyoscyamine sulfate monohydrate)

27 Cited Publications

Customer Review

Based on 27 publication(s) in Google Scholar

Atropine (Tropine tropate) sulfate monohydrate is a competitive muscarinic acetylcholine receptor (mAChR) antagonist with IC₅₀ values of 0.39 and 0.71 nM for Human mAChR M₄ and Chicken mAChR M₄, respectively. Atropine sulfate monohydrate inhibits ACh-induced relaxations in human pulmonary veins. Atropine sulfate monohydrate can be used for research of anti-myopia and bradycardia.

For research use only. We do not sell to patients.

Purity: 99.87%
CAS No.: 5908-99-6
Formula: C₁₇H₂₇NO₈S
Molecular Weight:347.43

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Atropine sulfate monohydrate Chemical Structure

CAS No.: 5908-99-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (27)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

mAChR4

All mAChR Isoforms :

In Vitro

Atropine (Tropine tropate; 1 μM; pulmonary veins and arteries) sulfate monohydrate inhibits ACh-induced relaxations in human pulmonary veins^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Atropine (Tropine tropate; 10 mg/kg; i.p.; once, for 40 minutes; Peromyscus sp.) sulfate monohydrate inhibits the cardiac arrhythmia which normally occurs throughout torpor^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

White-footed mice (Peromyscus sp.)^[2]

Dosage:

10 mg/kg

Administration:

Intraperitoneal injection; once, for 40 minutes

Result:

Increased heart rate was a decrease in cardiac arrhythmia.

Clinical Trial

NCT Number Sponsor Condition Start Date
Phase

A549

NCT01329991 Plexxikon| 2011-05 PHASE1

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 5908-99-6

Appearance Solid

Molecular Weight 347.43

Formula C₁₇H₂₇NO₈S

Color White to off-white

SMILES

O=[S](O)(O)=O.O=C(C(C1=CC=CC=C1)CO)O[C@H]2C[C@@H](CC3)N(C)[C@@H]3C2.O

Synonyms

Tropine tropate sulfate monohydrate; DL-Hyoscyamine sulfate monohydrate

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (27)

All Histological Imaging/StainingBio/Physico-chemical AssayWBIn Vivo Efficacy Study

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Journal Impact Factor

Journal Impact Factor

Most Recent

Cell

Cancer cells co-opt an inter-organ neuroimmune circuit to escape immune surveillance. [Abstract]

2025 Nov 26;188(24):6754-6773.e29. PMID: 41138728

Cell Metab

Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies. [Abstract]

2022 Dec 6;34(12):1999-2017.e10. PMID: 36395769

Cell Stem Cell

Human PSC-derived sinoatrial node-cardiac plexus assembloids model innervation-associated maturation of pacemaker systems. [Abstract]

2026 May 15:S1934-5909(26)00158-X. PMID: 42143017

Kidney Int

Parasympathetic nerves in the kidney pelvis contribute to blood pressure regulation. [Abstract]

2026 Jan 19:S0085-2538(26)00008-6. PMID: 41565024

Cell Discov

Adenosine-independent regulation of the sleep-wake cycle by astrocyte activity. [Abstract]

2023 Feb 7;9(1):16. PMID: 36746933

Neuron

Molecularly defined and functionally distinct cholinergic subnetworks. [Abstract]

2022 Nov 16;110(22):3774-3788.e7. PMID: 36130594

Sci Adv

EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia. [Abstract]

2025 Dec 5;11(49):eadw7972. PMID: 41348875

Small

A Balloon Electrochemical Sensor for Conformal Interfacing With Intestinal Wall and Real-Time Monitoring of Serotonin Release. [Abstract]

2026 Jan 21:e06988. PMID: 41563114

J Neuroinflammation

PVNOXT regulates acinar cell-mediated inflammatory response via DMVACh projections to the acinar cell. [Abstract]

2026 May 25. PMID: 42186091

J Hazard Mater

β-ionone inhibits the grazing of Daphnia sinensis by reducing the activity of acetylcholinesterase. [Abstract]

2024 Sep 5:479:135690. PMID: 39255669

J Hazard Mater

β-cyclocitral, a novel AChE inhibitor, contributes to the defense of Microcystis aeruginosa against Daphnia grazing. [Abstract]

2024 Mar 5:465:133248. PMID: 38147752

Food Chem

Screening of bioactive flavour compounds targeting muscarinic-3 acetylcholine receptor from Siraitia grosvenorii and evaluation of their synergistic anti-asthmatic activity. [Abstract]

2022 Nov 30:395:133593. PMID: 35792488

J Transl Med

Increased nerve density adversely affects outcome in colorectal cancer and denervation suppresses tumor growth. [Abstract]

2025 Jan 23;23(1):112. PMID: 39849539

Cell Rep

Nociceptive neurons protect cancer cells against oxidative stress. [Abstract]

2026 Mar 24;45(3):117086. PMID: 41831232

Cell Rep

Moderate heart rate reduction promotes cardiac regeneration through stimulation of the metabolic pattern switch. [Abstract]

2022 Mar 8;38(10):110468. PMID: 35263588

J Ethnopharmacol

Cholinergic signaling of muscarinic receptors directly involves in the neuroprotection of muscone by inducing Ca2+ antagonism and maintaining mitochondrial function. [Abstract]

2024 Jan 30;319(Pt 2):117192. PMID: 37734472

Atropine sulfate monohydrate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jan 30;319(Pt 2):117192. [Abstract]

Atropine (0.5 mg/kg; ip). Representative images of TTC-stained brain coronal sections of rats.

Atropine sulfate monohydrate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jan 30;319(Pt 2):117192. [Abstract]

Atropine (0.5 mg/kg; ip). Western blot results of signal pathways.

Atropine sulfate monohydrate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jan 30;319(Pt 2):117192. [Abstract]

Atropine (0.5 mg/kg; ip). Statistical results of neurological score by mNSS.

Atropine sulfate monohydrate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 Jan 30;319(Pt 2):117192. [Abstract]

Atropine (0.5 mg/kg; ip). Statistical results of Western blot in AKT/p-AKT.

Eur J Pharmacol

Activation of Piezo1 channels enhances spontaneous contractions of isolated human bladder strips via acetylcholine release from the mucosa. [Abstract]

2024 Sep 3:176954. PMID: 39237075

Atropine sulfate monohydrate purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Sep 3:176954. [Abstract]

Yoda1-induced ATP release was significantly attenuated in the presence of Atropine (1 μM).

Front Pharmacol

Eye Drops of Metformin Prevents Fibrosis After Glaucoma Filtration Surgery in Rats via Activating AMPK/Nrf2 Signaling Pathway. [Abstract]

2020 Jul 31;11:1038. PMID: 32903813

Am J Physiol Renal Physiol

Anatomic and functional evidence for renal autonomic innervation in normotensive and hypertensive rats. [Abstract]

2024 Nov 1;327(5):F885-F898. PMID: 39298550

Cardiovasc Drugs Ther

Transcutaneous Auricular Vagus Nerve Stimulation Ameliorates Heart Failure with Preserved Ejection Fraction Through Regulating Macrophage Polarization Mediated by Alpha7nAChR. [Abstract]

2025 Apr 7. PMID: 40193005

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Arch Oral Biol

Parasympathetic-macrophages-ductal epithelial cells axis promotes female rat submandibular gland regeneration after excretory duct ligation/deligation. [Abstract]

2023 Jan:145:105586. PMID: 36410162

Can J Physiol Pharmacol

Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro. [Abstract]

2020 Oct;98(10):725-732. PMID: 32516556

J Biosci

M1 macrophages-derived exosomes miR-34c-5p regulates interstitial cells of Cajal through targeting SCF. [Abstract]

2021:46:90. PMID: 34544909

Research Square Print

2023 Feb 7.

Research Square Print

December 16th, 2022.

Research Square Preprint

2022 Mar.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (143.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (95.93 mM; Need ultrasonic)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8783 mL

14.3914 mL

28.7828 mL

5 mM

0.5757 mL

2.8783 mL

5.7566 mL

10 mM

0.2878 mL

1.4391 mL

2.8783 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration Concentration

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Volume Volume

×
Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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×

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO    40% PEG300    5% Tween-80    45% Saline
Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Protocol 2

Add each solvent one by one: 10% DMSO    90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

Protocol 3

Add each solvent one by one: 10% DMSO    90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (287.83 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)

SDS (480 KB)

English - EN (480 KB)

Français - FR (480 KB)

Deutsch - DE (480 KB)

Norwegian - NO (480 KB)

Español - ES (480 KB)

Swedish - SV (480 KB)

Italian - IT (480 KB)

Korean - KR (480 KB)

Portuguese - PT (480 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. McBrien NA, et, al. How does atropine exert its anti-myopia effects? Ophthalmic Physiol Opt. 2013 May;33(3):373-8. [Content Brief]

[2]. Morhardt JE. Heart rates, breathing rates and the effects of atropine and acetylcholine on white-footed mice (Peromyscus sp.) during daily torpor. Comp Biochem Physiol. 1970 Mar 15;33(2):441-57. [Content Brief]

[3]. Carr BJ, et, al. Myopia-Inhibiting Concentrations of Muscarinic Receptor Antagonists Block Activation of Alpha2A-Adrenoceptors In Vitro. Invest Ophthalmol Vis Sci. 2018 Jun 1;59(7):2778-2791. [Content Brief]

[4]. Walch L, et, al. Evidence for a M(1) muscarinic receptor on the endothelium of human pulmonary veins. Br J Pharmacol. 2000 May;130(1):73-8. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

H₂O / DMSO 1 mM 2.8783 mL 14.3914 mL 28.7828 mL 71.9569 mL

5 mM 0.5757 mL 2.8783 mL 5.7566 mL 14.3914 mL

10 mM 0.2878 mL 1.4391 mL 2.8783 mL 7.1957 mL

15 mM 0.1919 mL 0.9594 mL 1.9189 mL 4.7971 mL

20 mM 0.1439 mL 0.7196 mL 1.4391 mL 3.5978 mL

25 mM 0.1151 mL 0.5757 mL 1.1513 mL 2.8783 mL

30 mM 0.0959 mL 0.4797 mL 0.9594 mL 2.3986 mL

40 mM 0.0720 mL 0.3598 mL 0.7196 mL 1.7989 mL

50 mM 0.0576 mL 0.2878 mL 0.5757 mL 1.4391 mL

60 mM 0.0480 mL 0.2399 mL 0.4797 mL 1.1993 mL

80 mM 0.0360 mL 0.1799 mL 0.3598 mL 0.8995 mL

DMSO 100 mM 0.0288 mL 0.1439 mL 0.2878 mL 0.7196 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Atropine sulfate monohydrate Related Classifications

HY-B0394 Related Classifications

GPCR/G Protein Neuronal Signaling

mAChR

Disease Areas

Neurological, Eye or Ear Disease Blood or Cardio-cerebrovascular Disease Cancer

Neurodegenerative Disease Cardiovascular Disease

Parkinson's Disease

Neurological Disease Cardiovascular Disease Cancer

Cancer Targeted Therapy Cancer Metabolism and Metastasis

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Keywords

Atropine sulfate
5908-99-6
Tropine tropate sulfate
DL-Hyoscyamine sulfate
mAChR
anti-myopia
tachyarrhythmias
Ach
pulmonary veins
bradycardia
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.8783 mL	14.3914 mL	28.7828 mL	71.9569 mL
5 mM	0.5757 mL	2.8783 mL	5.7566 mL	14.3914 mL
10 mM	0.2878 mL	1.4391 mL	2.8783 mL	7.1957 mL
15 mM	0.1919 mL	0.9594 mL	1.9189 mL	4.7971 mL
20 mM	0.1439 mL	0.7196 mL	1.4391 mL	3.5978 mL
25 mM	0.1151 mL	0.5757 mL	1.1513 mL	2.8783 mL
30 mM	0.0959 mL	0.4797 mL	0.9594 mL	2.3986 mL
40 mM	0.0720 mL	0.3598 mL	0.7196 mL	1.7989 mL
50 mM	0.0576 mL	0.2878 mL	0.5757 mL	1.4391 mL
60 mM	0.0480 mL	0.2399 mL	0.4797 mL	1.1993 mL
80 mM	0.0360 mL	0.1799 mL	0.3598 mL	0.8995 mL
DMSO	100 mM	0.0288 mL	0.1439 mL	0.2878 mL	0.7196 mL

Atropine sulfate monohydrate

27 Cited Publications

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Atropine sulfate monohydrate

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Publications Citing Use of MedChemExpress (MCE) Atropine sulfate monohydrate

All mAChR Isoforms

Biological Activity

Chemical Information

Publications (27)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Atropine sulfate monohydrate Related Classifications

HY-B0394 Related Classifications