Kasugamycin hydrochloride hydrate
Based on 1 publication(s) in Google Scholar
Kasugamycin (Ksg) hydrochloride hydrate is an antibiotic that binds to 30s and 70s ribosomes but not to the 50s subunit, and has anti-infective activity. Kasugamycin hydrochloride hydrate mimics mRNA nucleotides, disrupts tRNA binding and inhibits canonical translation initiation. Kasugamycin hydrochloride hydrate increases the sensitivity of mycobacteria to Rifampicin (HY-B0272) in vitro and in mouse infection models.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 200132-83-8
- Formula: C14H28ClN3O10
- Molecular Weight:433.84
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Kasugamycin hydrochloride hydrate
MoreAll Antibiotic Isoforms
More
Biological Activity
Kasugamycin (50-250 μg/mL) hydrochloride hydrate decreases mycobacterial mistranslation due to the indirect tRNA aminoacylation pathway[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Pharmacokinetic (PK) analysis in female BALB/c mice (8-10 weeks old)[3]
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Dosage:100 mg/kg, 200 mg/kg or 400 mg/kg (dissolved in 0.9% saline)
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Administration:Intramuscular injection, single dose
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Result:Showed dose-dependent concentration-time profiles in plasma, with rapid clearance.
400 mg/kg resulted in a Cmax/EC(mistranslation)50 of ~2.5 and time over EC50 of 1 hr or only 4% of the dosing interval.
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Animal Model:Mtb infection model in 6-week-old female BALB/c mice[3]
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Dosage:50 mg/kg and 400 mg/kg; with 10 mg/kg Rifampicin (orally gavage, once daily for 1 week)
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Administration:intraperitoneal injection, once daily for 1 weeks
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Result:Resulted in an astonishing 30-fold boosting of rifampicin killing of Mtb in mouse lungs.
Chemical Information
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CAS No. 200132-83-8
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Appearance Solid
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Molecular Weight 433.84
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Formula C14H28ClN3O10
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Color Off-white to light brown
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SMILES
OC(C(N[C@@H](C[C@@H]1N)[C@@H](C)O[C@]1([H])O[C@@H]2[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H]2O)=N)=O.Cl.O
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Synonyms
Ksg hydrochloride hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Front Mol Biosci
Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases. [Abstract]2021 Apr 7:8:640356. PMID: 33898519
Solvent & Solubility
H2O : 25 mg/mL (57.62 mM; Need ultrasonic)
DMSO : 1.67 mg/mL (3.85 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (19.20 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Schluenzen F, et al. The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation. Nat Struct Mol Biol. 2006 Oct;13(10):871-8. [Content Brief]
[2]. Schuwirth BS, et al. Structural analysis of kasugamycin inhibition of translation. Nat Struct Mol Biol. 2006 Oct;13(10):879-86. [Content Brief]
[3]. Chaudhuri S, et al. Kasugamycin potentiates rifampicin and limits emergence of resistance in Mycobacterium tuberculosis by specifically decreasing mycobacterial mistranslation. Elife. 2018 Aug 28;7:e36782. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.3050 mL | 11.5250 mL | 23.0500 mL | 57.6249 mL |
| H2O | 5 mM | 0.4610 mL | 2.3050 mL | 4.6100 mL | 11.5250 mL |
| 10 mM | 0.2305 mL | 1.1525 mL | 2.3050 mL | 5.7625 mL | |
| 15 mM | 0.1537 mL | 0.7683 mL | 1.5367 mL | 3.8417 mL | |
| 20 mM | 0.1152 mL | 0.5762 mL | 1.1525 mL | 2.8812 mL | |
| 25 mM | 0.0922 mL | 0.4610 mL | 0.9220 mL | 2.3050 mL | |
| 30 mM | 0.0768 mL | 0.3842 mL | 0.7683 mL | 1.9208 mL | |
| 40 mM | 0.0576 mL | 0.2881 mL | 0.5762 mL | 1.4406 mL | |
| 50 mM | 0.0461 mL | 0.2305 mL | 0.4610 mL | 1.1525 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Kasugamycin hydrochloride hydrate Related Classifications
HY-B1864B Related Classifications
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