Kasugamycin hydrochloride
Based on 1 publication(s) in Google Scholar
Kasugamycin (Ksg) hydrochloride is an aminoglycoside antibiotic (antibiotic) that binds to the bacterial 30S ribosomal subunit and inhibits canonical translation initiation. Kasugamycin hydrochloride binds to the P-site and E-site codon regions in the mRNA channel and interferes with mRNA-tRNA codon-anticodon interactions, thereby destabilizing initiator tRNA binding. Kasugamycin hydrochloride possesses anti-infective activity. Kasugamycin hydrochloride is used in research on bacterial translation and Pseudomonas infection.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 19408-46-9
- Formula: C14H26ClN3O9
- Molecular Weight:415.82
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-B1864AR
Kasugamycin hydrochloride (Standard) -
HY-18982
Anisomycin -
HY-N0626
Sorbic acid -
HY-W011522R
Taurolidine (Standard) -
HY-18982R
Anisomycin (Standard) -
HY-W011522
Taurolidine -
HY-N0626R
Sorbic acid (Standard) -
HY-130423
Milbemycin A3 -
HY-B0767
Spinosyn A -
HY-B1327A
Chlortetracycline -
HY-124920
Methiocarb -
HY-N10220
Paraherquamide E
Publications Citing Use of MedChemExpress (MCE) Kasugamycin hydrochloride
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Biological Activity
Description
In Vitro
Kasugamycin (Ksg) (100 mM) hydrochloride binds to the Thermus thermophilus 30S ribosomal subunit, forming two binding sites in the mRNA pathway; the primary binding site is located at the top of h44, between h24 and h28, and spans the P/E site region, while the secondary binding site is located adjacent to the E site and interacts with h23, h24 of 16S rRNA and ribosomal protein S7, with the Kasugamycin hydrochloride-30S complex structure determined at a resolution of 3.35 Å[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) hydrochloride inhibits the binding of MVF-mRNA to Escherichia coli 30S ribosomal subunits and 70S ribosomes, with inhibition rates of 40% and 25% at 1 mM, respectively[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) hydrochloride inhibits the binding of initiator fMet-tRNA to MVF-mRNA-programmed Escherichia coli 30S ribosomal subunits, with a maximum inhibition rate of 75% at 1 mM, while it has no significant effect on the binding of fMet-tRNA to 70S ribosomes[2].
Kasugamycin (500 μM; 15 min; 37 °C) hydrochloride inhibits the binding of leaderless mRNA and initiator fMet-tRNA to Escherichia coli 70S ribosomes by 29% and 13%, respectively, indicating that the 70S initiation complex formed by leaderless mRNA-fMet-tRNA is relatively insensitive to Kasugamycin hydrochloride[2].
Kasugamycin (12.5-50 μg/mL) hydrochloride inhibits 32 strains of Pseudomonas aeruginosa cultured in peptone water at pH 7.0, with 1 strain inhibited at 12.5 μg/mL, 19 strains inhibited at 25 μg/mL, and 12 strains inhibited at 50 μg/mL[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Kasugamycin (100 mg/kg; i.p.; single administration) hydrochloride achieves a 50% survival rate in mice infected intraperitoneally with mucin-containing Pseudomonas aeruginosa No. 87 (100 MLD)[4].
Kasugamycin (200 mg/kg; i.p.; single administration) hydrochloride achieves a 75% survival rate in mice infected intraperitoneally with mucin-containing Pseudomonas aeruginosa No. 87 (100 MLD)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:200 mg/kg
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Administration:i.p.; single injection
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Result:Resulted in 100% survival.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:100 mg/kg
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Administration:i.p.; single administration
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Result:Resulted in 50% survival.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:200 mg/kg
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Administration:i.p.; single administration
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Result:Resulted in 75% survival.
Chemical Information
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CAS No. 19408-46-9
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Appearance Solid
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Molecular Weight 415.82
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Formula C14H26ClN3O9
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Color Off-white to light yellow
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SMILES
OC(C(N[C@@H](C[C@@H]1N)[C@@H](C)O[C@]1([H])O[C@@H]2[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H]2O)=N)=O.[H]Cl
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Synonyms
Ksg hydrochloride
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Initial Source
Streptomyces kasugaensis
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Front Mol Biosci
Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases. [Abstract]2021 Apr 7:8:640356. PMID: 33898519
Solvent & Solubility
In Vitro:
H2O : 25 mg/mL (60.12 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang H, et al. Simultaneous determination of kasugamycin and validamycin-A residues in cereals by consecutive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment. 2018 Mar;35(3):487-497. [Content Brief]
[2]. Schluenzen F, et al. The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation. Nature structural & molecular biology. 2006 Oct;13(10):871-8. [Content Brief]
[3]. Kasuga K, et al. Heterologous production of kasugamycin, an aminoglycoside antibiotic from Streptomyces kasugaensis, in Streptomyces lividans and Rhodococcus erythropolis L-88 by constitutive expression of the biosynthetic gene cluster. Applied microbiology and biotechnology. 2017 May;101(10):4259-4268. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.4049 mL | 12.0244 mL | 24.0489 mL | 60.1222 mL |
| 5 mM | 0.4810 mL | 2.4049 mL | 4.8098 mL | 12.0244 mL | |
| 10 mM | 0.2405 mL | 1.2024 mL | 2.4049 mL | 6.0122 mL | |
| 15 mM | 0.1603 mL | 0.8016 mL | 1.6033 mL | 4.0081 mL | |
| 20 mM | 0.1202 mL | 0.6012 mL | 1.2024 mL | 3.0061 mL | |
| 25 mM | 0.0962 mL | 0.4810 mL | 0.9620 mL | 2.4049 mL | |
| 30 mM | 0.0802 mL | 0.4008 mL | 0.8016 mL | 2.0041 mL | |
| 40 mM | 0.0601 mL | 0.3006 mL | 0.6012 mL | 1.5031 mL | |
| 50 mM | 0.0481 mL | 0.2405 mL | 0.4810 mL | 1.2024 mL | |
| 60 mM | 0.0401 mL | 0.2004 mL | 0.4008 mL | 1.0020 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Kasugamycin hydrochloride Related Classifications
HY-B1864A Related Classifications
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