1. Immunology/Inflammation Apoptosis
  2. PD-1/PD-L1 Apoptosis
  3. BMS-1

BMS-1  (Synonyms: PD-1/PD-L1 inhibitor 1)

Cat. No.: HY-19991 Purity: 99.56%
COA Handling Instructions

BMS-1 is an inhibitor of the PD-1/PD-L1 protein/protein interaction (IC50 between 6 and 100 nM).

For research use only. We do not sell to patients.

BMS-1 Chemical Structure

BMS-1 Chemical Structure

CAS No. : 1675201-83-8

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 158 In-stock
Solution
10 mM * 1 mL in DMSO USD 158 In-stock
Solid
5 mg USD 144 In-stock
10 mg USD 228 In-stock
25 mg USD 456 In-stock
50 mg USD 660 In-stock
100 mg USD 1056 In-stock
200 mg   Get quote  
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Customer Review

Based on 22 publication(s) in Google Scholar

Top Publications Citing Use of Products

    BMS-1 purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2023 Feb 8.  [Abstract]

    BMS-1 (100 µg; Intrathecal injection; once every 2 days, for a total of four times) decreases the expression of SHP-1 in dorsal root ganglia of Male Sprague-Dawley (SD) rats.

    BMS-1 purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2023 Feb 8.  [Abstract]

    BMS-1 (50, 100 µg; Intrathecal injection; once every 2 days, for a total of four times) signifcantly reduces the expression of PD-L1 in CGRP+, neurons of Male Sprague-Dawley (SD) rats.

    BMS-1 purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2023 Feb 8.  [Abstract]

    BMS-1 (50, 100 µg; Intrathecal injection; once every 2 days, for a total of four times) signifcantly reduces the expression of PD-L1 in NF200+, neurons of Male Sprague-Dawley (SD) rats.

    BMS-1 purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2023 Feb 8.  [Abstract]

    BMS-1 (50, 100 µg; Intrathecal injection; once every 2 days, for a total of four times) signifcantly reduces the expression of PD-L1 in IB4+, neurons of Male Sprague-Dawley (SD) rats.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BMS-1 is an inhibitor of the PD-1/PD-L1 protein/protein interaction (IC50 between 6 and 100 nM)[1][2].

    IC50 & Target

    PD1-PDL1[1]

    In Vitro

    Since PD-1 mediated the exhaustion of natural killer (NK) cell by binding to its ligand PD-L1, BMS-1 (PD-1/PD-L1 inhibitor 1) (1 μM, 3 days) is used to disturb the interaction between PD-1 and PD-L1. Dexamethasone induced increase of PD-1 expression and decrease of cytotoxicity of the co-cultured NK92 cells are reversed by BMS-1[1]. BMS-1, a small-molecule immune checkpoint inhibitor of PD-1/PD-L1, can be used as a therapeutic strategy for tumor immunotherapy[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cytotoxicity Assay[1]

    Cell Line: NK cells and HepG2 cells
    Concentration: 1 μM
    Incubation Time: 3 days
    Result: Disturbed the interaction between PD-1 and PD-L1.
    In Vivo

    BMS-1 (500 μg/mL; 100 μL; i.p.) significantly increases the survival rates of the mVEGF165b group and mVEGF165b + MUC1 group[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c mice with EMT6 cells[3]
    Dosage: 500 μg/mL; 100 μL
    Administration: I.p.
    Result: Increased the survival rates of the mVEGF165b group and mVEGF165b + MUC1 group.
    mVEGF165b combined with MUC1 results significant retardation of the tumor growth.
    Molecular Weight

    475.58

    Formula

    C29H33NO5

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C([C@H]1N(CC2=C(OC)C=C(OCC3=C(C)C(C4=CC=CC=C4)=CC=C3)C=C2OC)CCCC1)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    Methanol : 25 mg/mL (52.57 mM; Need ultrasonic)

    DMSO : 7.14 mg/mL (15.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    DMF : 5 mg/mL (10.51 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1027 mL 10.5135 mL 21.0270 mL
    5 mM 0.4205 mL 2.1027 mL 4.2054 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (2.10 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1 mg/mL (2.10 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 10 mg/mL (21.03 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.56%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMF / DMSO / Methanol 1 mM 2.1027 mL 10.5135 mL 21.0270 mL 52.5674 mL
    5 mM 0.4205 mL 2.1027 mL 4.2054 mL 10.5135 mL
    10 mM 0.2103 mL 1.0513 mL 2.1027 mL 5.2567 mL
    DMSO / Methanol 15 mM 0.1402 mL 0.7009 mL 1.4018 mL 3.5045 mL
    Methanol 20 mM 0.1051 mL 0.5257 mL 1.0513 mL 2.6284 mL
    25 mM 0.0841 mL 0.4205 mL 0.8411 mL 2.1027 mL
    30 mM 0.0701 mL 0.3504 mL 0.7009 mL 1.7522 mL
    40 mM 0.0526 mL 0.2628 mL 0.5257 mL 1.3142 mL
    50 mM 0.0421 mL 0.2103 mL 0.4205 mL 1.0513 mL
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    BMS-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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