Cat. No.: HY-15349

Trovirdine

Name: Trovirdine
Brand: MedChemExpress
SKU: HY-15349
Price: 70.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: LY300046)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Trovirdine (LY300046) is a selective non-nucleoside HIV-1 reverse transcriptase inhibitor, with an IC₅₀ value of 0.012 μM against the wild-type enzyme. Trovirdine inhibits the replication of both wild-type and mutant HIV-1, and exerts synergistic or additive effects when combined with some antiviral drugs. Trovirdine is applicable to research related to HIV-1 infection.

For research use only. We do not sell to patients.

Purity : 98.62%
CAS No.: 149488-17-5
Formula: C₁₃H₁₃BrN₄S
Molecular Weight:337.24

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Trovirdine Chemical Structure

CAS No.: 149488-17-5

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

HIV-1

All HIV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MT4	IC50	0.013 μM	Inhibition of wild-type HIV-1 replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of wild-type HIV-1 replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	0.038 μM	90% inhibition of wild-type HIV-1 replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of wild-type HIV-1 replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	3.90 μM	Inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	19.0 μM	90% inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	24.5 μM	Inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	61.1 μM	90% inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	1.90 μM	Inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	5.80 μM	90% inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	0.019 μM	Inhibition of wild-type HIV-1 replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of wild-type HIV-1 replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	0.060 μM	90% inhibition of wild-type HIV-1 replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of wild-type HIV-1 replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	4.0 μM	Inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	34.8 μM	90% inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Ile100) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	13.7 μM	Inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	25.8 μM	90% inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Asn103) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC50	0.87 μM	Inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. Inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211
MT4	IC90	7.25 μM	90% inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent. 90% inhibition of HIV-1 (Cys181) mutant replication in MT-4 cells (in ddI combination context) assessed via cell viability measured by XTT assay after 6-day incubation with compound and virus, followed by 6-hour incubation with XTT reagent.	9341211

In Vitro

Trovirdine potently inhibits the activity of wild-type HIV-1 reverse transcriptase with an IC₅₀ of 0.012 μM; it also inhibits the replication of wild-type HIV-1 in MT-4 cells with an IC₅₀ of 0.013 μM^[1].
Trovirdine exhibits IC₅₀ values of 0.34 μM and 0.33 μM against HIV‑1 RT Tyr181→Cys and HIV‑1 RT Leu100→Ile mutants with 28‑fold and 27‑fold resistance respectively. In MT‑4 cells, Trovirdine shows IC₅₀ values of 3.90 μM, 24.5 μM and 1.90 μM for HIV‑1 Ile100, Asn103 and Cys181 mutant strains, with resistance factors >300‑fold, >1×10³‑fold and >140‑fold correspondingly^[1].

Trovirdine inhibits the activity of wild-type HIV‑1 reverse transcriptase (measured by the dCTP incorporation assay) with an IC₅₀ of 0.03 μM; it also inhibits the activities of the HIV‑1 RT Tyr181→Cys mutant and HIV‑1 RT Leu100→Ile mutant, with corresponding IC₅₀ values of 0.53 μM and 0.34 μM, respectively^[1].

Trovirdine (6 days) inhibits the replication of wild-type HIV-1 as well as HIV-1 Ile100, Asn103, and Cys181 mutants in MT-4 cells in the context of combination testing with ddI, with corresponding IC₅₀ values of 0.019 μM, 4.0 μM, 13.7 μM, and 0.87 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 149488-17-5
Appearance Solid
Molecular Weight 337.24
Formula C₁₃H₁₃BrN₄S
Color White to off-white
SMILES

S=C(NCCC1=NC=CC=C1)NC2=NC=C(Br)C=C2
Synonyms

LY300046
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

All

Sort by:

Journal Impact Factor

Int J Antimicrob Agents

Efficacy of antiretroviral compounds against Toxoplasma gondii in vitro. [Abstract]

2019 Dec;54(6):814-819. PMID: 31479744

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (296.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9652 mL

14.8262 mL

29.6525 mL

5 mM

0.5930 mL

2.9652 mL

5.9305 mL

10 mM

0.2965 mL

1.4826 mL

2.9652 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Villahermosa ML, et al. Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides. Biochemistry. 1997 Oct 28;36(43):13223-31.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9652 mL	14.8262 mL	29.6525 mL	74.1312 mL
	5 mM	0.5930 mL	2.9652 mL	5.9305 mL	14.8262 mL
	10 mM	0.2965 mL	1.4826 mL	2.9652 mL	7.4131 mL
	15 mM	0.1977 mL	0.9884 mL	1.9768 mL	4.9421 mL
	20 mM	0.1483 mL	0.7413 mL	1.4826 mL	3.7066 mL
	25 mM	0.1186 mL	0.5930 mL	1.1861 mL	2.9652 mL
	30 mM	0.0988 mL	0.4942 mL	0.9884 mL	2.4710 mL
	40 mM	0.0741 mL	0.3707 mL	0.7413 mL	1.8533 mL
	50 mM	0.0593 mL	0.2965 mL	0.5930 mL	1.4826 mL
	60 mM	0.0494 mL	0.2471 mL	0.4942 mL	1.2355 mL
	80 mM	0.0371 mL	0.1853 mL	0.3707 mL	0.9266 mL
	100 mM	0.0297 mL	0.1483 mL	0.2965 mL	0.7413 mL

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Trovirdine

Publications Citing Use of MedChemExpress (MCE) Trovirdine

All HIV Isoforms