1. Cell Cycle/DNA Damage
  2. HDAC


Cat. No.: HY-13428 Purity: 98.87%
Handling Instructions

Tubacin is a potent and selective inhibitor of HDAC6, with an IC50 value of 4 nM and approximately 350-fold selectivity over HDAC1.

For research use only. We do not sell to patients.
Tubacin Chemical Structure

Tubacin Chemical Structure

CAS No. : 537049-40-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 515 In-stock
1 mg USD 96 In-stock
5 mg USD 324 In-stock
10 mg USD 612 In-stock
20 mg USD 1164 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Tubacin is a potent and selective inhibitor of HDAC6, with an IC50 value of 4 nM and approximately 350-fold selectivity over HDAC1.

IC50 & Target

IC50: 4 nM (HDAC6), 1.27 μM (HDAC3), 1.27 μM (HDAC8), 1.40 μM (HDAC1), 3.35 μM (HDAC5), 3.71 μM (HDAC10), 3.79 μM (HDAC11), 4.31 μM (HDAC9), 6.27 μM (HDAC2), 9.7 μM (HDAC7), 17.3 μM (HDAC4)[1]

In Vitro

Tubacin preferentially induces α-tubulin hyperacetylation at a concentration of 2.5 µM, and induces α-tubulin acetylation at 5 µM and protects prostate cancer (LNCaP) cells from hydrogen peroxide-induced death at 8 µM via peroxiredoxin acetylation[1]. Tubacin (2.5 and 5 μM) specifically induces acetylation of α-tubulin in MM cells. Tubacin significantly inhibits both drug-sensitive and drug-resistant MM cell growth, with IC50 5-20 μM at 72 h. Tubacin also induces apoptosis by activation of caspases. Moreover, Tubacin inhibits binding of HDAC6 with dynein, and it induces significant accumulation of polyubiquitinated proteins, when combined with bortezomib. Tubacin and bortezomib induce synergistic antitumor activity in MM cell lines, and inhibits paracrine MM Cell Growth. Tubacin (5 μM) synergistically enhances bortezomib-induced cytotoxicity in patient MM cells without cytotoxicity to PBMCs[2]. Tubacin can concentration-dependently inhibits JEV-induced cytopathic effect and apoptosis, as well as reduces virus yield in human cerebellar medulloblastoma cells. The IC50 of virus yield is 0.26 μM for Tubacin. Tubacin also meaningfully blocks the production of intracellular infectious virus particles, with an IC50 of 1.52 μM. Tubacin induces the hyperacetylation of a HDAC6 substrate Hsp90 and reduces the interaction of Hsp90 with JEV NS5 protein[3].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.3853 mL 6.9266 mL 13.8531 mL
5 mM 0.2771 mL 1.3853 mL 2.7706 mL
10 mM 0.1385 mL 0.6927 mL 1.3853 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Tubacin is dissolved in DMSO.

HDAC inhibitors TSA, VPA, tubacin, and TBSA are used in the assay. Cytotoxicity of HDACi to TE671 and BHK-21 cells is evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 5 × 104 cells per well are seeded in 96-well plates and then treated with the indicated concentration of each HDACi. After 48-h of treatment, 25 μL of MTT solution (5 mg/mL) is added to each well and incubated at 37 °C with 5% CO2 for 3 h. After three washings with phosphate buffer saline (PBS), 100 μL DMSO is added into each well for dissolving formazan crystals. OD570−630 is measured by micro-ELISA reader and survival rate are calculated to indicate suppressive effects of each HDACi on the survival of TE671 and BHK-21 cells. Survival rate (%) = ((Acontrol − Aexperiment)/Acontrol) × 100%. 50% cytotoxic concentration (CC50) values are calculated by computer program[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







OCC(C=C1)=CC=C1[[email protected]@H]2C[[email protected]](CSC3=NC(C4=CC=CC=C4)=C(C5=CC=CC=C5)O3)O[[email protected]](C6=CC=C(NC(CCCCCCC(NO)=O)=O)C=C6)O2

Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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