Tubastatin A Hydrochloride
Based on 43 publication(s) in Google Scholar
Tubastatin A Hydrochloride (Tubastatin A HCl) is a potent and selective HDAC6 inhibitor with IC50 of 15 nM in a cell-free assay, and is selective (1000-fold more) against all other isozymes except HDAC8 (57-fold more). Tubastatin A Hydrochloride also inhibits HDAC10 and metallo-β-lactamase domain-containing protein 2 (MBLAC2).
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.31%
- CAS. Nr.: 1310693-92-5
- Formel: C20H22ClN3O2
- Molecular Weight:371.86
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Publications Citing Use of MedChemExpress (MCE) Tubastatin A Hydrochloride
More- Cell Host Microbe. 2026 Feb 11;34(2):245-262.e8. [Abstract]
- Nat Commun. 2026 Feb 5;17(1):1212. [Abstract]
- Acta Pharm Sin B. 2022 Oct;12(10):3891-3904. [Abstract]
- J Biomed Sci. 2025 Jan 20;32(1):9. [Abstract]
- Adv Sci (Weinh). 2025 Aug 7:e02624. [Abstract]
- Cell Death Differ. 2025 Jul 30. [Abstract]
- Cell Death Dis. 2022 Oct 21;13(10):888. [Abstract]
- NPJ Precis Oncol. 2024 Mar 7;8(1):66. [Abstract]
- Antioxidants (Basel). 2022 Apr 7;11(4):732. [Abstract]
- Antioxidants (Basel). 2020 Jul 9;9(7):599. [Abstract]
- Free Radic Biol Med. 2026 Oct:254:714-729.
- Free Radic Biol Med. 2025 Aug 13:240:59-70. [Abstract]
- Clin Transl Med. 2025 Jan;15(1):e70193. [Abstract]
- Neurotherapeutics. 2023 Jul;20(4):1215-1228. [Abstract]
- J Med Chem. 2024 Sep 12;67(17):15098-15117. [Abstract]
- J Med Chem. 2023 Dec 14;66(23):16075-16090. [Abstract]
- Atherosclerosis. 2021 Jan:317:1-9. [Abstract]
- Virulence. 2025 Dec;16(1):2495838. [Abstract]
- Mol Cancer Res. 2022 Jun 3;20(6):949-959. [Abstract]
- Mol Cancer Res. 2014 May;12(5):681-93. [Abstract]
- J Pathol. 2025 Apr 11. [Abstract]
- BMC Biol. 2018 Oct 18;16(1):116. [Abstract]
- Front Pharmacol. 2018 Feb 1:9:34. [Abstract]
- PLoS Pathog. 2021 Sep 20;17(9):e1009940. [Abstract]
- Am J Pathol. 2020 Dec;190(12):2376-2386. [Abstract]
- J Neurochem. 2026 Jun;170(6):e70493. [Abstract]
- J Dermatol Sci. 2022 Jun;106(3):181-188. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2020 May;1867(5):118676. [Abstract]
- J Nutr. 2020 Jul 1;150(7):1790-1798. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2026 Apr 18. [Abstract]
- Viruses. 2025 Jan 13;17(1):90. [Abstract]
- Int J Parasitol Drugs Drug Resist. 2026 May 17;31:100649.
- Hum Mol Genet. 2022 Jun 22;31(12):2035-2048. [Abstract]
- J Mol Neurosci. 2024 Mar 13;74(1):30. [Abstract]
- J Muscle Res Cell Motil. 2025 Nov 13. [Abstract]
- SSRN. 2026 May 21.
- bioRxiv. 2025 Nov 12.
- bioRxiv. 2025 Jul 12:2025.07.08.663754. [Abstract]
- bioRxiv. 2025 January 31.
- Aging (Albany NY). 2021 Mar 19;13(7):9820-9837. [Abstract]
- bioRxiv. 2021 Feb 25.
- Patent. US20180263995A1.
- University of Toronto. 2017 Nov.
Customer Validation & Images
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In Vivo Efficacy Study
Alle Beta-lactamase Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
HDAC6 15 nM (IC50) |
HDAC8 854 nM (IC50) |
HDAC1 16400 nM (IC50) |
In Vitro
Tubastatin A is substantially selective for all 11 HDAC isoforms and maintains over 1000-fold selectivity against all isoforms excluding HDAC8, where it has approximately 57-fold selectivity. In homocysteic acid (HCA) induced neurodegeneration assays, Tubastatin A displays dose-dependent protection against HCA-induced neuronal cell death starting at 5 μM with near complete protection at 10 μM[1]. At 100 ng/mL Tubastatin A increases Foxp3+ T-regulatory cells (Tregs) suppression of T cell proliferation in vitro[2]. Tubastatin A treatment in CC12 cells would lead to myotube formation impairment when alpha-tubulin is hyperacetylated early in the myogenic process; however, myotube elongation occurs when alpha-tubulin is hyeperacetylated in myotubes[3]. A recent study indicates that Tubastatin A treatment increases cell elasticity as revealed by atomic force microscopy (AFM) tests without exerting drastic changes to the actin microfilament or microtubule networks in mouse ovarian cancer cell lines, MOSE-E and MOSE-L[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1310693-92-5
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Appearance Solid
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Molecular Weight 371.86
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Formel C20H22ClN3O2
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Color White to yellow
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SMILES
O=C(C1=CC=C(C=C1)CN2C3=C(C4=C2C=CC=C4)CN(C)CC3)NO.[H]Cl
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Synonyms
Tubastatin A HCl; TSA HCl
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (43)
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Journal Impact Factor
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Most Recent
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Cell Host Microbe
Enterococcus faecalis-derived lactic acid suppresses macrophage activation to facilitate persistent and polymicrobial wound infections. [Abstract]2026 Feb 11;34(2):245-262.e8. PMID: 41605216 -
Nat Commun
The focal adhesion kinases regulate leptin action and the weight reducing effect of HDAC6 inhibition. [Abstract]2026 Feb 5;17(1):1212. PMID: 41644943 -
Acta Pharm Sin B
2022 Oct;12(10):3891-3904. PMID: 36213537
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Oct;12(10):3891-3904. [Abstract]
The baseline and blocking time-activity curves of PB118 in the mice whole brain. Compared with baseline mice, remarkably reduced radioactivity was observed in the brain of mice pretreated with Tubastatin A (3.0 mg/kg; 5 min before RI).
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J Biomed Sci
Targeting enolase 1 reverses bortezomib resistance in multiple myeloma through YWHAZ/Parkin axis. [Abstract]2025 Jan 20;32(1):9. PMID: 39828712 -
Adv Sci (Weinh)
The Probiotic Parabacteroides johnsonii Ameliorates Metabolic Disorders Through Promoting BCAAs to BSCFAs Conversion. [Abstract]2025 Aug 7:e02624. PMID: 40772426 -
Cell Death Differ
2025 Jul 30. PMID: 40739328 -
Cell Death Dis
HDAC6-dependent deacetylation of TAK1 enhances sIL-6R release to promote macrophage M2 polarization in colon cancer. [Abstract]2022 Oct 21;13(10):888. PMID: 36270986
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Oct 21;13(10):888. [Abstract]
Construction of HCT116 cell line with stable HDAC6 knockdown. Tubastatin A HCl is HDAC6 selective inhibitor.
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NPJ Precis Oncol
Connectivity mapping-based identification of pharmacological inhibitor targeting HDAC6 in aggressive pancreatic ductal adenocarcinoma. [Abstract]2024 Mar 7;8(1):66. PMID: 38454151 -
Antioxidants (Basel)
Sp1 S-Sulfhydration Induced by Hydrogen Sulfide Inhibits Inflammation via HDAC6/MyD88/NF-κB Signaling Pathway in Adjuvant-Induced Arthritis. [Abstract]2022 Apr 7;11(4):732. PMID: 35453416 -
Antioxidants (Basel)
Inhibition of HDAC6 Attenuates Diabetes-Induced Retinal Redox Imbalance and Microangiopathy. [Abstract]2020 Jul 9;9(7):599. PMID: 32660051 -
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Free Radic Biol Med
HDAC inhibition protects RPE cells from oxidative stress via enhanced mitochondrial fusion, cytoskeletal repair, and Nrf-2 activation. [Abstract]2025 Aug 13:240:59-70. PMID: 40816649 -
Clin Transl Med
Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition. [Abstract]2025 Jan;15(1):e70193. PMID: 39834092
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2025 Jan;15(1):e70193. [Abstract]
The inhibition of HDAC6 restored the spindle assembly caused by TUBB8 missense variants. Representative confocal images showed spindle morphology in groups of WT, D417N (DMSO), D417N + tubacin (2 μM), and D417N + Tubastatin A (10 μM; DMSO).
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Neurotherapeutics
2023 Jul;20(4):1215-1228. PMID: 37268847
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Neurotherapeutics. 2023 Jul;20(4):1215-1228. [Abstract]
Tubastatin A (TubA) (1 μM; overnight) treatment restores mitochondrial transport in cultured Gan−/− DRG neurons. Graph represents the significant increase in the total movement of mitochondria in Tubastatin A (TubA)-treated Gan−/− DRG neurons.
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Neurotherapeutics. 2023 Jul;20(4):1215-1228. [Abstract]
Tubastatin A (TubA) (1 μM; overnight) treatment increased levels of acetylated tubulin and Prph in the sciatic nerve of Gan−/−;TgPer mice. The figure represented the western blots for Nefl, Prph, acetyl tubulin, and α-tubulin, respectively, from the sciatic nerve of saline- and Tubastatin A (TubA)-treated mice.
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Neurotherapeutics. 2023 Jul;20(4):1215-1228. [Abstract]
Immunostaining of Nefl and Prph was performed on the cell body area of spinal neurons (larger than 250 μm²) from saline- and Tubastatin A (TubA)-treated mice, followed by quantification of the signal intensity. There was a significant decrease in the signal intensity for Nefl and Prph proteins in the perikaryon of spinal neurons from Gan−/−;TgPer mice treated with Tubastatin A (TubA) when compared to saline-treated Gan−/−;TgPer mice.
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J Med Chem
Discovery of a Novel Benzimidazole Derivative Targeting Histone Deacetylase to Induce Ferroptosis and Trigger Immunogenic Cell Death. [Abstract]2024 Sep 12;67(17):15098-15117. PMID: 39145486 -
J Med Chem
Development and Pharmacochemical Characterization Discover a Novel Brain-Permeable HDAC11-Selective Inhibitor with Therapeutic Potential by Regulating Neuroinflammation in Mice. [Abstract]2023 Dec 14;66(23):16075-16090. PMID: 37972387 -
Atherosclerosis
Targeting HDAC6 attenuates nicotine-induced macrophage pyroptosis via NF-κB/NLRP3 pathway. [Abstract]2021 Jan:317:1-9. PMID: 33321327 -
Virulence
The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication. [Abstract]2025 Dec;16(1):2495838. PMID: 40275702 -
Mol Cancer Res
Constitutive β-Catenin Overexpression Represses Lncrna MIR100HG Transcription via HDAC6-Mediated Histone Modification in Colorectal Cancer. [Abstract]2022 Jun 3;20(6):949-959. PMID: 35247921 -
Mol Cancer Res
NEDD9 regulates actin dynamics through cortactin deacetylation in an AURKA/HDAC6-dependent manner. [Abstract]2014 May;12(5):681-93. PMID: 24574519 -
J Pathol
Histone deacetylase 6 inhibition attenuates pathological cardiac hypertrophy by promoting autophagy through MAP1LC3B ubiquitination. [Abstract]2025 Apr 11. PMID: 40212005 -
BMC Biol
Microtubule polyglutamylation and acetylation drive microtubule dynamics critical for platelet formation. [Abstract]2018 Oct 18;16(1):116. PMID: 30336771 -
Front Pharmacol
HDAC6 Inhibition Promotes Transcription Factor EB Activation and Is Protective in Experimental Kidney Disease. [Abstract]2018 Feb 1:9:34. PMID: 29449811
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Feb 1:9:34. [Abstract]
HDAC6 inhibition with Tubastatin A acetylates TFEB, facilitates TFEB nuclear translocation and attenuates programmed cell death in NRK-52E cells. Immunoblotting for acetylated α-tubulin in NRK-52E cells treated with Tubastatin A.
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PLoS Pathog
PCV2 targets cGAS to inhibit type I interferon induction to promote other DNA virus infection. [Abstract]2021 Sep 20;17(9):e1009940. PMID: 34543359 -
Am J Pathol
Inhibition of Histone Deacetylase 6 by Tubastatin A Attenuates the Progress of Osteoarthritis via Improving Mitochondrial Function. [Abstract]2020 Dec;190(12):2376-2386. PMID: 32926854 -
J Neurochem
Effects of Lysine Deacetylation Inhibition Alone or in Combination With Arimoclomol on TDP-43 Proteinopathy. [Abstract]2026 Jun;170(6):e70493. PMID: 42283221 -
J Dermatol Sci
2022 Jun;106(3):181-188. PMID: 35637111 -
Biochim Biophys Acta Mol Cell Res
Shear stress stimulates integrin β1 trafficking and increases directional migration of cancer cells via promoting deacetylation of microtubules. [Abstract]2020 May;1867(5):118676. PMID: 32044386 -
J Nutr
Dietary Glucose Increases Glucose Absorption and Lipid Deposition via SGLT1/2 Signaling and Acetylated ChREBP in the Intestine and Isolated Intestinal Epithelial Cells of Yellow Catfish. [Abstract]2020 Jul 1;150(7):1790-1798. PMID: 32470978
Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: J Nutr. 2020 Jul 1;150(7):1790-1798. [Abstract]
Effects of TBSA (Tubastatin A) pretreatment on glucose-induced changes in mRNA amounts of genes in IECs of yellow catfish.
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Naunyn Schmiedebergs Arch Pharmacol
Aucubin inhibits epithelial-mesenchymal transition and angiogenesis in colorectal cancer via the HDAC6/PI3K/Akt signaling pathway. [Abstract]2026 Apr 18. PMID: 41999432 -
Viruses
2025 Jan 13;17(1):90. PMID: 39861880 -
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Hum Mol Genet
E3 ligase Smurf1 protects against misfolded SOD1 in neuronal cells by promoting its K63 ubiquitylation and aggresome formation. [Abstract]2022 Jun 22;31(12):2035-2048. PMID: 35022748 -
J Mol Neurosci
Effects of the Glucocorticoid-Mediated Mitochondrial Translocation of Glucocorticoid Receptors on Oxidative Stress and Pyroptosis in BV-2 Microglia. [Abstract]2024 Mar 13;74(1):30. PMID: 38478195 -
J Muscle Res Cell Motil
Tubastatin A attenuates impaired autophagic degradation and promotes myogenic program in skeletal muscle following downhill running. [Abstract]2025 Nov 13. PMID: 41233637 -
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bioRxiv
2025 Jul 12:2025.07.08.663754. PMID: 40672312 -
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Aging (Albany NY)
Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy. [Abstract]2021 Mar 19;13(7):9820-9837. PMID: 33744850 -
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Tubastatin A Hydrochloride purchased from MedChemExpress. Usage Cited in: University of Toronto. 2017 Nov.
Tubastatin A induces a dose-dependent increase in acetylated α-tubulin levels in NRK-52E cells.
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 11.67 mg/mL (31.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 6.67 mg/mL (17.94 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 1% DMSO 99% Saline
Solubility: ≥ 0.52 mg/mL (1.40 mM); Clear solution
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Kinase Assay[1]
Enzyme inhibition assays are performed using the Reaction Biology HDAC Spectrum platform. The HDAC1, 2, 4, 5, 6, 7, 8, 9, 10, and 11 assays use isolated recombinant human protein; HDAC3/NcoR2 complex is used for the HDAC3 assay. Substrate for HDAC1, 2, 3, 6, 10, and 11 assays is a fluorogenic peptide from p53 residues 379-382 (RHKKAc); substrate for HDAC8 is fluorogenic diacyl peptide based on residues 379-382 of p53 (RHKAcKAc). Acetyl-Lys (trifluoroacetyl)-AMC substrate is used for HDAC4, 5, 7, and 9 assays. Tubastatin A is dissolved in DMSO and tested in 10-dose IC50 mode with 3-fold serial dilution starting at 30 μM. Control Compound Trichostatin A (TSA) is tested in a 10-dose IC50 with 3-fold serial dilution starting at 5 μM. IC50 values are extracted by curve-fitting the dose/response slopes.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay[1]
Primary cortical neuron cultures are obtained from the cerebral cortex of fetal Sprague-Dawley rats (embryonic day 17). All experiments are initiated 24 hours after plating. Under these conditions, the cells are not susceptible to glutamate-mediated excitotoxicity. For cytotoxicity studies, cells are rinsed with warm PBS and then placed in minimum essential medium containing 5.5 g/L glucose, 10% fetal calf serum, 2 mM L-glutamine, and 100 μM cystine. Oxidative stress is induced by the addition of the glutamate analogue homocysteate (HCA; 5 mM) to the media. HCA is diluted from 100-fold concentrated solutions that are adjusted to pH 7.5. In combination with HCA, neurons are treated with Tubastatin A at the indicated concentrations. Viability is assessed after 24 hours by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration[2]
The effects of HDAC6 targeting in dextran sodium sulfate (DSS) and adoptive transfer models of colitis are evaluated, using 10 mice per group. Freshly prepared 4% (wt/vol) DSS is added daily for 5 days to the pH-balanced tap water of WT B6 mice. Mice are treated daily for 7 days with tubacin or niltubacin (0.5 mg/kg of body weight/day, i.p.), and colitis is assessed by daily monitoring of body weight, stool consistency, and fecal blood. Stool consistency is scored as 0 (hard), 2 (soft), or 4 (diarrhea), and fecal blood (Hemoccult) is scored as 0 (absent), 2 (occult), or 4 (gross). To assess prevention of colitis in a T cell-dependent model, CD4+ CD45RBhi T cells (1×106) isolated from WT mice using magnetic beads (>95% cell purity, flow cytometry) are injected i.p. into B6/Rag1−/− mice plus CD4+ CD25+ Tregs (1.25×105) isolated using magnetic beads from HDAC6−/− or WT mice (>90% Treg purity, flow cytometry) and mice are monitored biweekly for clinical evidence of colitis. To assess therapy of established T cell-dependent colitis, B6/Rag1−/− mice are injected i.p. with CD4+ CD45RBhi cells (1×106). Once colitis has developed, mice also receive CD4+ CD25+ Tregs (5×105 cells) isolated as described above from HDAC6−/− or WT mice or treatment with HDAC6i (tubastatin A) or HSP90i (17-AAG). Mice are monitored for continued weight loss and stool consistency. At the cessation of the study, paraffin sections of colons stained with Alcian Blue or hematoxylin and eosin are graded histologically or evaluated by immunoperoxidase staining for Foxp3+ Treg infiltration.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (287 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Kyle V. Butler et al. Rational Design and Simple Chemistry Yield a Superior, Neuroprotective HDAC6 Inhibitor, Tubastatin A J. Am. Chem. Soc., 2010, 132 (31), pp 10842-10846 [Content Brief]
[2]. de Zoeten EF, et al. Histone deacetylase 6 and heat shock protein 90 control the functions of Foxp3(+) T-regulatory cells. Mol Cell Biol. 2011 May;31(10):2066-78. [Content Brief]
[3]. Di Fulvio S, et al. Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation. PLoS One. 2011;6(12):e28563 [Content Brief]
[4]. Ketene AN, et al. Actin filaments play a primary role for structural integrity and viscoelastic response in cells. Integr Biol (Camb). 2012 May;4(5):540-9. [Content Brief]
[5]. Brijmohan AS, et al. HDAC6 Inhibition Promotes Transcription Factor EB Activation and Is Protective in Experimental Kidney Disease. Front Pharmacol. 2018 Feb 1;9:34. [Content Brief]
[6]. Severin Lechner, et al. Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target. Nat Chem Biol. 2022 Apr 28. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.6892 mL | 13.4459 mL | 26.8918 mL | 67.2296 mL |
| 5 mM | 0.5378 mL | 2.6892 mL | 5.3784 mL | 13.4459 mL | |
| 10 mM | 0.2689 mL | 1.3446 mL | 2.6892 mL | 6.7230 mL | |
| 15 mM | 0.1793 mL | 0.8964 mL | 1.7928 mL | 4.4820 mL | |
| DMSO | 20 mM | 0.1345 mL | 0.6723 mL | 1.3446 mL | 3.3615 mL |
| 25 mM | 0.1076 mL | 0.5378 mL | 1.0757 mL | 2.6892 mL | |
| 30 mM | 0.0896 mL | 0.4482 mL | 0.8964 mL | 2.2410 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Tubastatin A Hydrochloride Related Classifications
HY-13271 Related Classifications
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