AHR agonist 3
Based on 1 Customer Validation
AHR agonist 3 is an aryl hydrocarbon receptor (AhR) agonist, that can induces cell cycle arrest or apoptosis via activation of tumor-suppressive transcriptional programs. AHR agonist 3 inhibits triple-negative breast cancer (TNBC) stem cell growth via AhR while exhibits minimal cytotoxicity against normal human primary cells and can be used for cancer research.
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- Reinheit : 99.79%
- CAS. Nr.: 23749-58-8
- Formel: C18H10N2O
- Molecular Weight:270.28
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
AHR agonist 3 (Analog 523) (10 nΜ; 24 hour or 2-3 weeks) induces cell cycle arrest and inhibits clonogenicity in AhR WT and AhR KO MDA-MB-468 cells [1].
AHR agonist 3 (0-10 μΜ; 48-72 hours) drives AhR-dependent apoptosis and growth inhibition in breast cancerous cells, but not normal breast epithelial cells or on-tumorigenic cells and inhibits TNBC stem cell growth via AhR.[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:AhR WT and AhR KO MDA-MB-468 cells
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Concentration:10 nM
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Incubation Time:24 hours or 2−3 weeks
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Result:Induced an AhR-dependent S+G2/M phase arrest for Short-term (24 h) treatment.
Completely exhibited the clonogenicity of AhR expressing cells while exhibiting a minimal impact on AhR deficient cells.
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Cell Line:MDA-MB-468 cells, MCF10A, HEK293T and normal primary human fibroblast
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Concentration:0-10 μΜ
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Incubation Time:48-72 hours
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Result:Human mammary epithelial cells (HMECs), nonmalignant mammary epithelial line Induced AhR-dependent apoptosis and growth inhibition in cancerous but not normal cells.
Inhibited TNBC stem cell growth via AhR.
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- Protocol for Cell Cycle
- CCK-8/WST-8 Cell Proliferation Assay
- Cell Counting-Based Growth Curve Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Cell Cytotoxicity Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
Chemical Information
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CAS. Nr. 23749-58-8
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Appearance Solid
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Molecular Weight 270.28
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Formel C18H10N2O
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Color Light yellow to green yellow
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SMILES
C1=CC=C2C(=C1)N=C3C4=CC=CC5=C4C(=CC=C5)C(=O)N23
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 10 mg/mL (37.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.6999 mL | 18.4993 mL | 36.9987 mL | 92.4967 mL |
| 5 mM | 0.7400 mL | 3.6999 mL | 7.3997 mL | 18.4993 mL | |
| 10 mM | 0.3700 mL | 1.8499 mL | 3.6999 mL | 9.2497 mL | |
| 15 mM | 0.2467 mL | 1.2333 mL | 2.4666 mL | 6.1664 mL | |
| 20 mM | 0.1850 mL | 0.9250 mL | 1.8499 mL | 4.6248 mL | |
| 25 mM | 0.1480 mL | 0.7400 mL | 1.4799 mL | 3.6999 mL | |
| 30 mM | 0.1233 mL | 0.6166 mL | 1.2333 mL | 3.0832 mL |
AHR agonist 3 Related Classifications
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