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Cat. No.: HY-E71730

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AlkB (D135S), Nuclease-free

Name: AlkB (D135S), Nuclease-free
Brand: MedChemExpress
SKU: HY-E71730
Price: 1300.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: RNA m1G Demethylase (D135S))

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AlkB (D135S), Nuclease-free (RNA m1G Demethylase (D135S)) is a nuclease-free N1-methylguanosine (m1G) demethylase that specifically removes the methyl group from m1G residues in small non-coding RNAs. AlkB (D135S), Nuclease-free effectively promotes the reverse transcription of small non-coding RNAs and supports high-throughput sequencing methods such as CPA-seq. AlkB (D135S), Nuclease-free can be used for the precise detection and quantitative analysis of methylated small non-coding RNAs that are easily missed by conventional methods.

For research use only. We do not sell to patients.

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

AlkB (D135S), Nuclease-free Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

In Vitro

When used in combination with AlkB, AlkB (D135S), Nuclease-free efficiently demethylates m¹G in small RNAs (sRNAs) from HEK293T cells, human mesenchymal stem cells, primary human hepatocytes, and 9 types of mouse tissues, enabling comprehensive analysis of previously undiscovered sRNA populations via CPA-seq^[1].
AlkB (D135S) (0.04 μg/μl; 2 h) efficiently demethylates m¹G, m¹A and m³C modifications in small RNAs derived from R1/E mouse embryonic stem cells (mESCs), while preserving intact m⁷G modifications to support downstream reverse transcription and sequencing^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Appearance Liquid
Color Colorless to light yellow
SMILES

[AlkB (D135S), Nuclease-free]
Synonyms

RNA m1G Demethylase (D135S)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (265 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang H, et al. CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini. Cell discovery. 2021 Apr 19;7(1):25. [Content Brief]

[2]. Lin S, et al. Nucleotide resolution profiling of mG tRNA modification by TRAC-Seq. Nature protocols. 2019 Nov;14(11):3220-3242. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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