Anti-Mouse CD3E Antibody (500A2)

Anti-Mouse CD3E Antibody (500A2) co-immunoprecipitates TCR β homodimers from SCB.24 and SCB.29 immature pre-T cells, and TCR αβ heterodimers from B6.2.16BW mature T cells at a concentration of 1.96 mg/mL^[1].
Anti-Mouse CD3E Antibody (500A2) induces rapid, robust, and transient intracellular calcium flux in SCB.29 immature pre-T cells at saturating concentrations^[1].
Anti-Mouse CD3E Antibody (500A2) uniformly stains thymocytes from F23.1⁺ TCR β transgenic Scid mice at saturating concentrations, confirming the expression of CD3E on the surface of these cells^[1].
Anti-Mouse CD3E Antibody (500A2) stains immature pro-T cells transfected with SCB.29 TCR β at saturation concentration, but fails to stain untransfected Sci/ET27F immature pro-T cells, demonstrating that surface expression of CD3E depends on TCR β^[1].
Anti-Mouse CD3E Antibody (500A2) specifically binds to splenocytes and thymocytes from C57BL/6 mice that express CD3ε^[2].
Anti-Mouse CD3E Antibody (500A2) activates naive mouse T cells in vitro, and the anti-CD4 Fab fragment blocks the activation of mouse T cell clones by this soluble form^[2].
HRP-conjugated Anti-Mouse CD3E Antibody (500A2) (10 μg/mL; 1 h at 4°C) mediates the biotinylation of clustered TCRαβ in 2C T cells via the EMARS reaction, confirming the association of TCRαβ with CD3ε in the T cell receptor complex^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.