Anti-Mouse/Human CD44 Antibody (IM7)
Based on 3 publication(s) in Google Scholar
Anti-Mouse/Human CD44 Antibody (IM7) is an anti-huamn and mouse CD44 IgG2b monoclonal antibody that recognizes a conserved epitope outside the HA-binding domain of the CD44 molecule. Anti-Mouse/Human CD44 Antibody (IM7) exerts a potent anti-inflammatory effect by inducing the shedding of cell surface CD44, significantly improving symptoms in mice with rheumatoid arthritis without affecting relevant immune responses. Anti-Mouse/Human CD44 Antibody (IM7) can be used for researches on inflammation conditions and cancer such as arthritis and osteosarcoma.
For research use only. We do not sell to patients.
- Purity: 99.07%
- Molecular Weight:150 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Anti-Mouse/Human CD44 Antibody (IM7)
More
Customer Validation & Images
-
In Vivo Efficacy Study
-
Histological Imaging/Staining
-
WB
-
RT-PCR
-
Flow Cytometry
Biological Activity
Rat IgG2b kappa
Human/Mouse
CD44
Anti-Mouse/Human CD44 Antibody (IM7) (0.05-50 μg/mL, 24-72 h) significantly reduces cell activity of SW1353 cells in a time-dependent manner[1].
Anti-Mouse/Human CD44 Antibody (IM7) (50 μg/mL, 72 h) significantly induces cell apoptosis of SW1353 cells[1].
Anti-Mouse/Human CD44 Antibody (IM7) (12-20 h) inhibits the migration and adhesion of inflammatory cells by inducing CD44 shedding and blocking hyaluronic acid binding in white blood cells and synovial cells from arthritis mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:SW1353 cells
-
Concentration:0.05, 5 and 50 μg/mL
-
Incubation Time:24, 48 and 72 h
-
Result:Significantly reduced the activity of SW1353 cells after 72 hours.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:BALB/c mice with proteoglycan-arthritis and DBA/1 mice with collagen-arthritis[2]
-
Dosage:Initial dose of 100 µg, maintenance dose of 50 µg
-
Administration:Intraperitoneal injection (i.p.), once daily, for 10 days
-
Result:Significantly reduced joint swelling within 24-36 hours.
Restored normal joint function in mice after 8 days of treatment.
Significantly reduced leukocyte infiltration in synovium.
Prevented white blood cells from migrating to synovial tissue, but did not affect their homing to lymph nodes.
NP_001034240.1
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vivo CD44 neutralization; in vitro CD44 neutralization
-
Immobilized CD44 Protein, Mouse (HEK293, His, HY-P74291) can bind Anti-Mouse CD44 Antibody (IM7). The EC50 for this effect is 0.165 μg/mL.
Chemical Information
-
Appearance Liquid
-
Molecular Weight 150 kDa
-
Color Colorless to light yellow
-
SMILES
[Anti-Mouse/Human CD44 Antibody (IM7)]
-
Shipping
Shipping with dry ice.
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
-
Journal Impact Factor
-
Most Recent
-
JCI Insight
Versican regulating viscoelasticity drives pleural fibrosis via mechanotransductive signaling. [Abstract]2026 Apr 23:e199507. PMID: 42024449 -
Cell Signal
CD44-mediated copper accumulation drives Ly6Chi macrophages activation in ulcerative colitis. [Abstract]2025 Dec 11:112315. PMID: 41389969
Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315. [Abstract]
Anti-Mouse/Human CD44 Antibody (IM7) (100 μL; i.p.; 7 d) significantly attenuated body weight loss, reduced disease activity index (DAI) scores, and partially restored colon length in UC (ulcerative colitis) mice.
Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315. [Abstract]
Anti-Mouse/Human CD44 Antibody (IM7) (100 μL; i.p.; 7 d) repaired mucosal epithelial barriers, diminished inflammatory infiltration, and improved histopathology scores in colon tissues of UC (ulcerative colitis) mice.
Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315. [Abstract]
Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) reduced CD44 protein levels and elevated ATP7A expression in BMDM-derived Ly6Chi macrophages.
Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315. [Abstract]
Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) reduced mRNA levels of pro-inflammatory cytokines IL-6 and TNF-α in BMDM-derived Ly6Chi macrophages.
Anti-Mouse/Human CD44 Antibody (IM7) purchased from MedChemExpress. Usage Cited in: Cell Signal. 2025 Dec 11:112315. [Abstract]
Anti-Mouse/Human CD44 Antibody (IM7) (10 μg/mL) diminished the ROS proportion in BMDM-derived Ly6Chi macrophages.
-
Purity & Documentation
-
Data Sheet (263 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yoshida M, et al. Induction of apoptosis by anti-CD44 antibody in human chondrosarcoma cell line SW1353. Biomed Res. 2008 Feb;29(1):47-52. [Content Brief]
[2]. Mikecz K, et al. Anti-CD44 treatment abrogates tissue oedema and leukocyte infiltration in murine arthritis. Nat Med. 1995 Jun;1(6):558-63. [Content Brief]
[3]. Mikecz K, et al. Modulation of hyaluronan receptor (CD44) function in vivo in a murine model of rheumatoid arthritis. Arthritis Rheum. 1999 Apr;42(4):659-68. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse/Human CD44 Antibody (IM7) Related Classifications
HY-P99126 Related Classifications
Powered by Bioz