Anti-Mouse IL-12 p35 Antibody (C18.2)
Based on 1 Customer Validation
Anti-Mouse IL-12 p35 Antibody (C18.2) is a rat-derived IgG2a κ type antibody inhibitor, targeting to mouse IL-12 p35. Anti-Mouse IL-12 p35 Antibody (C18.2) can neutralize IL-12. Anti-Mouse IL-12 p35 Antibody (C18.2) can be used for the researches of infection, inflammation and immunology, such as airway inflammation and F. tularensis infection.
For research use only. We do not sell to patients.
- Purity : 95%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
IL-12 p35
In Vitro
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse IL-12 p35 Antibody (C18.2) (500 μg, i.p., twice at days -1, 0, and 1 after infection) reduces the frequency and number of effector memory CD4+ T cells in both the lungs and spleens in F. tularensis live vaccine strain infected mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ovalbumin-induced allergic airway inflammation mice models[1]
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Dosage:250 μg
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Administration:Intraperitoneally injection, at days 33 and 37, 2 h before the ovalbumin challenges
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Result:Decreased the airway hyper responsiveness and eosinophil and lymphocyte counts in the BAL fluid.
Reduced ovalbumin -specific IgG2a levels and CD3+CD4+ cells.
Gene ID
Accession
P43431 & P43432
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo IL-12 p35 neutralization; in vitro IL-12 p35 neutralization; ELISA; Immunoprecipitation
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse IL-12 p35 Antibody (C18.2)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Meyts I, et al. IL-12 contributes to allergen-induced airway inflammation in experimental asthma. J Immunol. 2006 Nov 1;177(9):6460-70. [Content Brief]
[2]. Bitsaktsis C, et al. In vivo mechanisms involved in enhanced protection utilizing an Fc receptor-targeted mucosal vaccine platform in a bacterial vaccine and challenge model. Infect Immun. 2015 Jan;83(1):77-89. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse IL-12 p35 Antibody (C18.2) Related Classifications
HY-P990226 Related Classifications
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