Anti-Mouse IL-2 Antibody (S4B6-1)
Based on 1 Customer Validation
Anti-Mouse IL-2 Antibody (S4B6-1) is an anti-mouse IL-2 IgG2a monoclonal antibody. Anti-Mouse IL-2 Antibody (S4B6-1) can reduce CD4+ T cells and increase Tregs. Anti-Mouse IL-2 Antibody (S4B6-1) can induce Th17 cell differentiation. Anti-Mouse IL-2 Antibody (S4B6-1) can be used for research on cancer such as melanoma. The recommend isotype control of Anti-Mouse IL-2 Antibody (S4B6-1): Rat IgG2a kappa, Isotype Control (HY-P990679).
For research use only. We do not sell to patients.
- Purity : 96.97%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
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IL-2 |
In Vitro
In Vivo
Anti-Mouse IL-2 Antibody (S4B6-1) (150 μg, i.p., once every 3 days, for 2 weeks) reduces the number of central Treg cells (cTR) in C57BL/6 mice bearing B16.Flt3L tumors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5×105 B16F10 cells injected female C57BL/6J mice (8 weeks)[1]
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Dosage:100 µg, combined with IL-2 mAb (JES6-1A12)
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Administration:Intraperitoneal injection (i.p.), once every 3 days
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Result:Significantly inhibited the expansion of natural killer (NK) cells in tumors.
Accelerated tumor growth and reduced survival rate.
Reduced activation phenotype of host CD4+ T cells (CD25+Foxp3-) in tumors.
Increased Tregs (CD25+ Foxp3+) in tumors.
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Animal Model:3×106 B16.Flt3L cells injected C57BL/6 mice[3]
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Dosage:150 μg
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Administration:Intraperitoneal injection (i.p.), once every 3 days, for 2 weeks
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Result:Significantly reduced cTR cells without affecting effector Treg cells (eTR).
Inhibited Stat5 phosphorylation.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo IL-2 neutralization; in vivo IL-2 receptor stimulation (as a complex with IL-2)
Verified Bioactivity
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Immobilized His-DTA-mIL2 can bind Anti-Mouse IL-2 Antibody (S4B6-1). The EC50 for this effect is 59.8 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse IL-2 Antibody (S4B6-1)]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Dang N, et al. Donor Lymphocyte-Derived Natural Killer Cells Control MHC Class I-Negative Melanoma. Cancer Immunol Res. 2020 Jun;8(6):756-768. [Content Brief]
[2]. Li M, et al. Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice. Genomics Proteomics Bioinformatics. 2022 Apr;20(2):288-303. [Content Brief]
[3]. Smigiel KS, et al. CCR7 provides localized access to IL-2 and defines homeostatically distinct regulatory T cell subsets. J Exp Med. 2014 Jan 13;211(1):121-36. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse IL-2 Antibody (S4B6-1) Related Classifications
HY-P990796 Related Classifications
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