GRB10 Antibody (YA7893)(PBS only)

Cat. No.: HY-P88209A: Data Sheet User Guide for Antibodies
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GRB10 Antibody (YA7893) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to GRB10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GRB10 Antibody (YA7893) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to GRB10.

Host

Mouse

Molecular Weight

Predicted band size: 67.1 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13322

Immunogen

Human recombinant protein fragment corresponding to amino acids 164-415 of human GRB10 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain

Isoforms & Post-Translational Modification：Q13322 has four isomers: Q13322-1: 67231 Da (predicted); Q13322-2: 62026 Da (predicted); Q13322-3: 60848 Da (predicted); Q13322-4: 66487 Da (predicted).
Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation (By similarity)

Subunit：Interacts with ligand-activated tyrosine kinase receptors, including FGFR1, INSR, IGF1R, MET and PDGFRB in a phosphotyrosine-dependent manner through the SH2 domain (By similarity)

Synonyms

Grb-10; GRB-IR; IRBP; MEG1; RSS

Documentation

User Guide for Antibodies (1077 KB)