PPP1R8 Antibody (YA8283)

Cat. No.: HY-P88599: Ficha de datos User Guide for Antibodies
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PPP1R8 Antibody (YA8283) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPP1R8.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

PPP1R8 Antibody (YA8283) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPP1R8.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 22.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q12972

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-225 of human PPP1R8 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation|Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing

Subcellular Localization：Nucleus,Nucleus speckle,Cytoplasm

Expression：
Tissue_Specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells

Isoforms & Post-Translational Modification：Q12972 has three isomers: Q12972-1: 38479 Da (predicted); Q12972-2: 22711 Da (predicted); Q12972-3: 13344 Da (predicted).
May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity)

Subunit：Interacts with phosphorylated CDC5L, SF3B1 and MELK

Synonyms

ARD-1; ARD1; NIPP-1; NIPP1; PRO2047

Documentación

User Guide for Antibodies (1077 KB)