Aza-CyBz
Based on 1 Customer Validation
Aza-CyBz is a NaCl-responsive near-infrared heptamethine cyanine fluorescent probe. Aza-CyBz has an excitation wavelength of 740 nm and detects signals in the range of 760-900 nm. NaCl induces Aza-CyBz to form ordered aggregates, resulting in fluorescence quenching. Aza-CyBz shows no obvious response to interfering substances such as plant carbohydrates, hormones, PEG and ROS. Aza-CyBz can enter plant mitochondria and reflect changes in their salt concentration, and can be used for visual imaging of salt stress in Arabidopsis thaliana root tips and living plants.
For research use only. We do not sell to patients.
- Purity : 95.21%
- Formula: C43H49ClIN3O2
- Molecular Weight:802.23
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
Aza-CyBz (10 μM) exhibits a sensitive and selective fluorescent response to Na+Cl− in cell-free solution, with a limit of detection of 170 μM. Na+Cl− induces the formation of J-aggregates, which quenches the fluorescence of monomers[1].
Aza-CyBz (10 μM; 1 h) enables visualization of salt stress in Arabidopsis thaliana root tips, with its fluorescence intensity decreasing as NaCl concentration increases; mitochondrial colocalization experiments demonstrate that this probe can enter mitochondria and reflect changes in salt concentration within them[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
Appearance Solid
-
Molecular Weight 802.23
-
Formula C43H49ClIN3O2
-
Color Brown to dark brown
-
SMILES
ClC1=C(/C=C/C2=[N+](CCC)C(C=CC=C3)=C3C2(C)C)CN(C(OCC4=CC=CC=C4)=O)C/C1=C\C=C5C(C)(C)C(C=CC=C6)=C6N/5CCC.[I-]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Protocols
-
ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Aza-CyBz Related Classifications
HY-D2864 Related Classifications
Powered by Bioz