Cat. No.: HY-W010253

Benzylurea

Customer Review

Based on 1 Customer Validation

Benzylurea is an anti-inflammatory agent. Benzylurea inhibits LPS (HY-D1056)-induced upregulation of MTCH2 expression and regulates pathways associated with mitochondrial function, inflammation and cell survival. Benzylurea alleviates LPS-induced proliferation inhibition and apoptosis of periodontal ligament fibroblasts, as well as the release of proinflammatory cytokines. Benzylurea can be used in studies related to periodontitis.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 538-32-9
Formula: C₈H₁₀N₂O
Molecular Weight:150.18

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Benzylurea Chemical Structure

CAS No.: 538-32-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

IL-1β

IL-6

NLRP3

All Interleukin Related Isoforms :

All NF-κB Isoforms :

All Bcl-2 Family Isoforms :

All NOD-like Receptor (NLR) Isoforms :

All Cytochrome P450 Isoforms :

In Vitro

Benzylurea (0-120 μM; 24 h) protects human periodontal ligament fibroblasts (hPDLFs) against LPS (HY-D1056)-induced loss of viability, and exhibits low intrinsic cytotoxicity to hPDLFs at the concentration of 10 μM; whereas higher concentrations (20-120 μM; 24 h) reduce the viability of hPDLFs in a dose-dependent manner^[1].
Benzylurea (10 μM; 24 h) inhibits the secretion of IL-1β, IL-6 and TNF-α induced by LPS in human periodontal ligament fibroblasts (hPDLFs), and blocks the nuclear translocation of NF-κB p65^[1].
Benzylurea (10 μM; 24 h) restores the mitochondrial membrane potential of human periodontal ligament fibroblasts (hPDLFs) damaged by LPS and inhibits the production of ROS^[1].
Benzylurea (10 μM; 24 h) can restructure and restore the F-actin cytoskeleton in human periodontal ligament fibroblasts (hPDLFs) damaged by LPS^[1].
Benzylurea (10 μM; 24 h) regulates protein expression in hPDLFs by reducing LPS-induced levels of MTCH2, NF-κB p65, Bax, IL-1β, IL-8 and NLRP3, while increasing the LPS-suppressed levels of CYB5B and BCL2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

human periodontal ligament fibroblasts (hPDLFs)
Concentration:

10 μM
Incubation Time:

24 h
Result:

Significantly inhibited the LPS-induced elevation of IL-1β, IL-6, and TNF-α levels in hPDLF culture supernatants.

Chemical Information

CAS No. 538-32-9
Appearance Solid
Molecular Weight 150.18
Formula C₈H₁₀N₂O
Color White to off-white
SMILES

O=C(N)NCC1=CC=CC=C1
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (665.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.6587 mL

33.2934 mL

66.5868 mL

5 mM

1.3317 mL

6.6587 mL

13.3174 mL

10 mM

0.6659 mL

3.3293 mL

6.6587 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu L, et al. Benzylurea Protects hPDLFs Against LPS-Induced Mitochondrial Dysfunction Through MTCH2. Oral Dis. 2025;31(4):1255-1267. [Content Brief]

[2]. Khalil AT, et al. Benzylamides from Salvadora persica. Arch Pharm Res. 2006;29(11):952-956. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.6587 mL	33.2934 mL	66.5868 mL	166.4669 mL
	5 mM	1.3317 mL	6.6587 mL	13.3174 mL	33.2934 mL
	10 mM	0.6659 mL	3.3293 mL	6.6587 mL	16.6467 mL
	15 mM	0.4439 mL	2.2196 mL	4.4391 mL	11.0978 mL
	20 mM	0.3329 mL	1.6647 mL	3.3293 mL	8.3233 mL
	25 mM	0.2663 mL	1.3317 mL	2.6635 mL	6.6587 mL
	30 mM	0.2220 mL	1.1098 mL	2.2196 mL	5.5489 mL
	40 mM	0.1665 mL	0.8323 mL	1.6647 mL	4.1617 mL
	50 mM	0.1332 mL	0.6659 mL	1.3317 mL	3.3293 mL
	60 mM	0.1110 mL	0.5549 mL	1.1098 mL	2.7744 mL
	80 mM	0.0832 mL	0.4162 mL	0.8323 mL	2.0808 mL
	100 mM	0.0666 mL	0.3329 mL	0.6659 mL	1.6647 mL

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Benzylurea

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Angiogenesis-Related Compound Library

Mitochondria-Targeted Compound Library

Transcription Factor-Targeted Library

Plant-Sourced Natural Product Library

Pain-Related Compound Library

Metabolic Enzyme Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cytokine Inhibitors Library

Cell Death Library

Multi-Target Compound Library

Radioprotector Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Structurally Diverse Fragment Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Rheumatic Arthritis Compound Library

Pattern Recognition Receptors Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All Interleukin Related Isoforms

All NF-κB Isoforms

All Bcl-2 Family Isoforms

All NOD-like Receptor (NLR) Isoforms

All Cytochrome P450 Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Benzylurea Related Classifications

Cat. No.: HY-W010253