X-press Tag Peptide
Based on 1 Customer Validation
X-press Tag Peptide is a tag peptide used for protein purification. X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide.
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- Pureté : 98.03%
- CAS No.: 339988-16-8
- Formule: C41H59N9O20
- Masse moléculaire:997.96
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Stockage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Activité biologique
Description
In Vitro
X-press Tag is a commonly used protein purification tag. X-press Tag binds to the N-terminus or C-terminus of the target protein, affinity chromatography will be performed using specific antibodies or ligands to efficiently separate the target protein from other cellular components[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 339988-16-8
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Appearance Solid
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Masse moléculaire 997.96
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Formule C41H59N9O20
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Color White to off-white
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Sequence
Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys
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Sequence Shortening
DLYDDDDK
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvant et solubilité
In Vitro:
H2O : ≥ 100 mg/mL (100.20 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0020 mL | 5.0102 mL | 10.0204 mL | 25.0511 mL |
| 5 mM | 0.2004 mL | 1.0020 mL | 2.0041 mL | 5.0102 mL | |
| 10 mM | 0.1002 mL | 0.5010 mL | 1.0020 mL | 2.5051 mL | |
| 15 mM | 0.0668 mL | 0.3340 mL | 0.6680 mL | 1.6701 mL | |
| 20 mM | 0.0501 mL | 0.2505 mL | 0.5010 mL | 1.2526 mL | |
| 25 mM | 0.0401 mL | 0.2004 mL | 0.4008 mL | 1.0020 mL | |
| 30 mM | 0.0334 mL | 0.1670 mL | 0.3340 mL | 0.8350 mL | |
| 40 mM | 0.0251 mL | 0.1253 mL | 0.2505 mL | 0.6263 mL | |
| 50 mM | 0.0200 mL | 0.1002 mL | 0.2004 mL | 0.5010 mL | |
| 60 mM | 0.0167 mL | 0.0835 mL | 0.1670 mL | 0.4175 mL | |
| 80 mM | 0.0125 mL | 0.0626 mL | 0.1253 mL | 0.3131 mL | |
| 100 mM | 0.0100 mL | 0.0501 mL | 0.1002 mL | 0.2505 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.