Cat. No.: HY-N0704

Agrimol B

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Based on 1 publication(s) in Google Scholar

Agrimol B, a polyphenol, is an orally active and potent SIRT1 activator. Agrimol B shows anti-adipogenic and anticancer activity. Agrimol B shows antibacterial activity against plant pathogens. Agrimol B dramatically inhibits 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. The action of Agrimol B on the cancer cells is likely derived from its effect on c-MYC, SKP2 and p27.

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Pureté: 99.50%
CAS No.: 55576-66-4
Formule: C₃₇H₄₆O₁₂
Masse moléculaire:682.75

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Agrimol B Chemical Structure

CAS No.: 55576-66-4

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Publications Citing Use of MedChemExpress (MCE) Agrimol B

Biochem Pharmacol. 2024 Jul 24:116453. [Abstract]

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Cell Proliferation/Viability AssayIFWB

Cell Proliferation/Viability Assay
IF
WB

Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

SIRT1

PPARγ

Voir tous les produits spécifiques à Isoform Sirtuin :

Voir tous les produits spécifiques à Isoform PPAR :

In Vitro

Agrimol B blocks adipogenesis at the early stage of differentiation in a dose-dependent manner, with an IC₅₀ of 3.35 ± 0.32 μM^[1].
? Agrimol B induces SIRT1 (silent information regulator 2 homolog 1) translocation and expression in 3T3-L1 adipocytes^[1].
? Agrimol B inhibits PC-3 and A549 cells growth, with GI50 (growth inhibition 50%) values of 29 and 19 μM, GI₇₅ values of 49 and 50 μM, and GI90 values of 63 and 76 μM, respectively^[2].
? Agrimol B (0-76 μM) dose-dependently increases cells at G0 in both cell lines^[2].
? Agrimol B (0-76 μM, 3 days) reduces the protein expression of c-MYC and SKP2 (S-phase kinase-associated protein 2), increases p27 (cyclin-dependent kinase inhibitor 1B), and down-regulates SPT16 (Suppressor of Ty Homolog-16) and SSRP1 (Structure-Specific Recognition Protein 1)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[2]

Cell Line:

PC-3 and A549 cells
Concentration:

29 and 63 μM (PC-3), 19 and 76 μM (A549)
Incubation Time:

10 min
Result:

Dose-dependently increased cells at G0 in both cell lines. Reduced percentage of cells positive for Ki-67, and increased p27 positive cell population in PC-3 and A549 cells.

Western Blot Analysis^[2]

Cell Line:

PC-3 and A549 cells
Concentration:

0, 29, 49, 63 μM (PC-3); 0, 19, 50, and 76 μM (A549)
Incubation Time:

3 days
Result:

Reduced c-MYC, SKP2 and increased p27 in both cell lines, and down-regulated SPT16 and SSRP1 in A549 cells with no effect on CRM1 in both cell lines.

Immunofluorescence^[1]

Cell Line:

3T3-L1 preadipocyte
Concentration:

0, 3, and 10 μM
Incubation Time:

6 days
Result:

Significantly increased nuclear positive rate of SIRT1; Markedly increased SIRT1 expression at 10 μM, the effect vanished at 3 μM. Down-regulated PPARγ and C/EBPα (CCAAT/enhancer-binding protein α) expression; Significantly decreased FAS (fatty acid synthesis), UCP-1 (uncoupling protein-1), and apoE (apolipoprotein E) expression at 10 μM.

In Vivo

Agrimol B (10 mg/kg, Orally, daily) reduces growth of prostate cancer cell xenograft in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice (6-week-old, male, injected with PC-3 cells in 0.2 mL PBS subcutaneously)^[2]
Dosage:

10 mg/kg
Administration:

Orally, daily, after 15 days of cancer cell implantation
Result:

Inhibited tumor growth in a mouse model of human prostate cancer, reduced the tumor volume at day 31 and day 32.

Chemical Information

CAS No. 55576-66-4
Appearance Solid
Masse moléculaire 682.75
Formule C₃₇H₄₆O₁₂
Color White to yellow
SMILES

CC[C@H](C)C(C1=C(O)C(CC2=C(O)C(C(CCC)=O)=C(OC)C(C)=C2O)=C(O)C(CC3=C(O)C(C(CCC)=O)=C(OC)C(C)=C3O)=C1O)=O
Structure Classification
- Phenols
- Polyphenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

All Cell Proliferation/Viability AssayIFWB

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Journal Impact Factor

Biochem Pharmacol

Activating the iNOS regulatory pathway by arginine deprivation targets energy metabolism to induce autophagy-dependent apoptosis against spinal echinococcosis. [Abstract]

2024 Jul 24:116453. PMID: 39059773

Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453. [Abstract]

Cytotoxicity was texted by CKK8 assay treated with Agrimol B (20, 50, 100 μM).

Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453. [Abstract]

JC-1 fluorescent staining of PSCs after 3 days treated with 50 µM, 100 µM of Agrimol B.

Agrimol B purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jul 24:116453. [Abstract]

Western blotting showed LC3, p62 activation in PSCs cells treated with Agrimol B (25, 50, 100 μM) in a time-dose dependent manner.

Solvant et solubilité

In Vitro:

DMSO : 5 mg/mL (7.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4647 mL

7.3233 mL

14.6467 mL

5 mM

0.2929 mL

1.4647 mL

2.9293 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.5 mg/mL (0.73 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (0.73 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (283 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Agrimol B, et al. Agrimol B suppresses adipogenesis through modulation of SIRT1-PPAR gamma signal pathway. Biochem Biophys Res Commun. 2016 Aug 26;477(3):454-60. [Content Brief]

[2]. Hnit SST, et al. Agrimol B present in Agrimonia pilosa Ledeb impedes cell cycle progression of cancer cells through G0 state arrest. Biomed Pharmacother. 2021 Sep;141:111795. [Content Brief]

[3]. Chun S B, et al. Antibacterial Activities against Plant Pathogens and Identification of Agrimol B from Agrimonia pilosa LEDEB[J]. 농약과학회지 제10권 3호, 2006, 10(3):230-236.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4647 mL	7.3233 mL	14.6466 mL	36.6166 mL
DMSO	5 mM	0.2929 mL	1.4647 mL	2.9293 mL	7.3233 mL

Agrimol B

1 Cited Publications

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Agrimol B

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Publications Citing Use of MedChemExpress (MCE) Agrimol B

Voir tous les produits spécifiques à Isoform Sirtuin

Voir tous les produits spécifiques à Isoform PPAR

Activité biologique

Chemical Information

Publications (1)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Agrimol B Related Classifications

HY-N0704 Related Classifications