Obacunone

Name: Obacunone
Brand: MedChemExpress
SKU: HY-N0428
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0428 Purity: 99.75%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Obacunone is a highly oxidized triterpenoid limonoid isolated from citrus plants. Obacunone exerts its anticancer effects by inducing apoptosis. Obacunone also protects retinal pigment epithelial (RPE) cells from ultraviolet (UV) radiation (UVR)-induced oxidative damage.

For research use only. We do not sell to patients.

CAS No. : 751-03-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Obacunone:

Obacunone (Standard) In-stock

4 Publications Citing Use of MCE Obacunone

Cell Proliferation/Viability Assay

RT-PCR

Histological Imaging/Staining

: Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]; CCK-8 assay was used to measure the viability of HK-2 cells treatment with Obacunone (OB) solutions at different concentrations (0, 5, 10, 20, 40, 80 and 160 µM).

: Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]; Levels of ROS in HK-2 cells from each group were measured using immunofluorescence treated with Obacunone (OB) (20, 40, 80 μM).

: Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]; Protein levels of GPX4, ACSL4, SLC7A11 and FTH1 in HK-2 cells from each group were measured using western blotting treated with Obacunone (OB) (20, 40, 80 μM).

: Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]; The expression levels of Nrf2 and KEAP1 in HK-2 cells from the Control, HG, HG + Obacunone (OB) 20 µM, HG + OB 40 µM and HG + OB 80 µM groups were assessed using reverse transcription-quantitative PCR.

: Obacunone purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]; Severity of renal pathological damage in each group of rats was observed using hematoxylin and eosin staining treated with Obacunone (OB) (25, 50, 100 mg/kg, p.o.).

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
J774.A1	IC₅₀	83.61 μM Compound: 5	Antiinflammatory activity in mouse J774A1 cells assessed as reduction of LPS-induced nitric oxide production treated 2 hrs before LPS addition measured after 20 hrs by using Griess reagent Antiinflammatory activity in mouse J774A1 cells assessed as reduction of LPS-induced nitric oxide production treated 2 hrs before LPS addition measured after 20 hrs by using Griess reagent	[PMID: 23688954]
MCF7	IC₅₀	0.02 μM Compound: 11g	Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay Antiproliferative activity against human MCF7 cells incubated for 48 hrs by [3H]-thymidine incorporation assay	[PMID: 33289552]
MDA-MB-435	IC₅₀	6.8 μM Compound: 11g	Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay Antiproliferative activity against human MDA-MB-435 cells incubated for 48 hrs by [3H]-thymidine incorporation assay	[PMID: 33289552]
SW480	IC₅₀	56 μM Compound: 11g	Antiproliferative activity against human SW480 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human SW480 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]

In Vitro

Obacunone (1-50 μM; 48 h) effectively inhibits UVR-induced cytotoxicity^[2].
Obacunone (25 μM; 1 h+12 h) improves UVR-induced oxidative damage in ARPE-19 cells and inhibits reactive oxygen species accumulation, mitochondrial depolarization, lipid peroxidation, and single-stranded DNA accumulation in human ARPE-19 cells^[2].
Obacunone (25 μM; 1 h+12 h) slows UVR-induced apoptosis in human ARPE-19 cells and murine RPE cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Obacunone (10 mg/kg; ip; single dose) inhibits Bleomycin (HY-108345)-induced lung inflammation and fibrosis in B6 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

454.51

Formula

C₂₆H₃₀O₇

CAS No.

751-03-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(OC1(C)C)C=C[C@]([C@@]1([H])C2)(C)[C@@]3([H])CC[C@]4(C)[C@@]([C@H]5C(O[C@H]4C6=COC=C6)=O)(O5)[C@]3(C)C2=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (220.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2002 mL	11.0009 mL	22.0017 mL
5 mM	0.4400 mL	2.2002 mL	4.4003 mL
10 mM	0.2200 mL	1.1001 mL	2.2002 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (260 KB) HNMR (147 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Chidambara Murthy KN, et al. Obacunone and obacunone glucoside inhibit human colon cancer (SW480) cells by the induction of apoptosis. Food Chem Toxicol. 2011 Jul;49(7):1616-25. [Content Brief]

[2]. Huang DR, et al. Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury. Aging (Albany NY). 2021 Feb 1;13(8):11010-11025. [Content Brief]

[3]. Xu S, et al. Obacunone activates the Nrf2-dependent antioxidant responses. Protein Cell. 2016 Sep;7(9):684-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2002 mL	11.0009 mL	22.0017 mL	55.0043 mL
	5 mM	0.4400 mL	2.2002 mL	4.4003 mL	11.0009 mL
	10 mM	0.2200 mL	1.1001 mL	2.2002 mL	5.5004 mL
	15 mM	0.1467 mL	0.7334 mL	1.4668 mL	3.6670 mL
	20 mM	0.1100 mL	0.5500 mL	1.1001 mL	2.7502 mL
	25 mM	0.0880 mL	0.4400 mL	0.8801 mL	2.2002 mL
	30 mM	0.0733 mL	0.3667 mL	0.7334 mL	1.8335 mL
	40 mM	0.0550 mL	0.2750 mL	0.5500 mL	1.3751 mL
	50 mM	0.0440 mL	0.2200 mL	0.4400 mL	1.1001 mL
	60 mM	0.0367 mL	0.1833 mL	0.3667 mL	0.9167 mL
	80 mM	0.0275 mL	0.1375 mL	0.2750 mL	0.6876 mL
	100 mM	0.0220 mL	0.1100 mL	0.2200 mL	0.5500 mL

Obacunone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Obacunone751-03-1ApoptosisNrf2 signalingUV radiationobacunoneoxidative injuryretinal pigment epithelium cellsInhibitorinhibitorinhibit

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Obacunone

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4 Publications Citing Use of MCE Obacunone

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Complete Stock Solution Preparation Table

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