AHMT
Based on 1 Customer Validation
AHMT is an aldehyde-specific and aldehyde-reactive colorimetric detection reagent. AHMT detects the formation of Phenylacetaldehyde in high-throughput screening for engineered anti-Markovnikov oxidation activity. AHMT serves as a formaldehyde detection reagent to detect formaldehyde produced in the Methanethiol oxidase reaction.
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- Pureté : 98.0%
- CAS No.: 1750-12-5
- Formule: C2H6N6S
- Masse moléculaire:146.17
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Stockage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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AHMT (Standard)Reference Standard -
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Activité biologique
Description
In Vitro
The AHMT-based anti-Markovnikov HTS assay detects phenylacetaldehyde produced from the oxidation of styrene by CglUPO variants secreted by S. cerevisiae, enabling the screening of a mutant library comprising 670 clones[1].
The AHMT assay detects methanethiol-derived formaldehyde at 549 nm, revealing copper-dependent HCHO generation by recombinant RdMTO and SELENBP1[2].
AHMT reacts with formaldehyde under alkaline conditions to form a product detectable at 549 nm, which is suitable for recombinant proteins but not for liver lysates[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1750-12-5
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Appearance Solid
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Masse moléculaire 146.17
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Formule C2H6N6S
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Color White to off-white
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SMILES
S=C(N/1N)NNC1=N\N
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvant et solubilité
In Vitro:
DMSO : 4 mg/mL (27.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Research Protocol for Drug Screening technologies
Drug screening technologies are experimental and computational strategies used to identify small molecules or chemical probes that modulate a defined molecular target, signaling pathway, cellular phenotype, disease model, or patient-derived response profile. High-throughput screening tests many compounds in miniaturized assay formats, while quantitative high-throughput screening tests compounds across concentration ranges so that potency and efficacy can be inferred from concentration-response behavior rather than from a single-point signal. The core biological function of a drug-screening strategy is to connect compound exposure with measurable pathway activity, target modulation, cell-state change, viability, cytotoxicity, morphology, or disease-relevant phenotype. Assay performance must be evaluated before screening because hit identification depends on the separation between positive and negative controls, control variability, plate effects, outliers, and the statistical framework
Pureté et documentation
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Fiche technique (274 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.8413 mL | 34.2067 mL | 68.4135 mL | 171.0337 mL |
| 5 mM | 1.3683 mL | 6.8413 mL | 13.6827 mL | 34.2067 mL | |
| 10 mM | 0.6841 mL | 3.4207 mL | 6.8413 mL | 17.1034 mL | |
| 15 mM | 0.4561 mL | 2.2804 mL | 4.5609 mL | 11.4022 mL | |
| 20 mM | 0.3421 mL | 1.7103 mL | 3.4207 mL | 8.5517 mL | |
| 25 mM | 0.2737 mL | 1.3683 mL | 2.7365 mL | 6.8413 mL |
AHMT Related Classifications
HY-D0906 Related Classifications
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