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Cat. No.: HY-158269

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T4-BSA

Name: T4-BSA
Brand: MedChemExpress
SKU: HY-158269
Price: 344.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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T4-BSA is an antigen-adjuvant conjugate of T4 (HY-18341) conjugated to bovine serum albumin (BSA). T4-BSA can be immobilized on a solid-phase carrier, thereby enabling specific binding to free T4 antibodies or their variable regions. T4-BSA can be used for biopanning and ELISA experiments of anti-T4 antibodies.

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Stockage:

Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

T4-BSA Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

T4-BSA (10 μg/mL; 30 min-overnight) functions in the biopanning system to capture T4-specific antibody fragments (Fab) from a phage display library, and two clones, D11 and F11, are screened and obtained as T4-specific binders^[1].
T4-BSA (1 μg/mL; overnight) specifically binds to T4 phage antibodies, with IC₅₀ values of 5.3 ng/mL and 12.8 ng/mL for binding to D11 and F11, respectively^[1].
T4-BSA (1 μg/mL; overnight) is not recognized by anti-TBBPA VHH T3-15 or VHH-AP fusion in ELISA binding assays^[3].
T4-BSA (0.02-2.00 μg/well) supports only low binding of mAb 1, 9, and 62 in cell-free ELISA, 50-100 times lower than the hormonogenic sites of hTg^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Enzyme-Linked Immunosorbent Assay (ELISA)

Chemical Information

Appearance Liquid
Color Light yellow to light brown
SMILES

[T4-BSA]
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (279 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Islam KN, et al. Direct construction of an open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4. Analytical chemistry. 2011 Feb 01;83(3):1008-14.

[2]. Wani TA, et al. Analytical Application of Flow Immunosensor in Detection of Thyroxine and Triiodothyronine in Serum. Assay and drug development technologies. 2016 Nov;14(9):535-542.

[3]. Wang J, et al. One-step immunoassay for tetrabromobisphenol a using a camelid single domain antibody-alkaline phosphatase fusion protein. Analytical chemistry. 2015;87(9):4741-8.

[4]. BAETS MD, et al. Monoclonal antibodies to human thyroglobulin as probes for thyroglobulin structure. Endocrinology. 1987 Mar 1;120(3):1104-11.

[5]. Bellisario R, et al. Simultaneous measurement of thyroxine and thyrotropin from newborn dried blood-spot specimens using a multiplexed fluorescent microsphere immunoassay. Clinical Chemistry. 2000 Sep 1;46(9):1422-4.

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

Minimum order quantity

100 mg

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