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Cat. No.: HY-115999

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Carbonic anhydrase inhibitor 12

Carbonic anhydrase inhibitor 12 is a potent CA II inhibitor, also has inhibitory activity in CA I (K_is of 1.72 and 271 nM in CA II and CA I, respectively). Carbonic anhydrase inhibitor 12 has potent anticancer activity against different cancer cell lines.

For research use only. We do not sell to patients.

CAS No.: 2883451-38-3
Formula: C₂₇H₂₂BrN₅O₅S₂
Molecular Weight:640.53

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Carbonic anhydrase inhibitor 12 Chemical Structure

CAS No.: 2883451-38-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

CA Ⅱ

All Carbonic Anhydrase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	16.5 μM Compound: 14h	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
COLO 205	IC₅₀	13.3 μM Compound: 14h	Antiproliferative activity against human COLA 205 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human COLA 205 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
HCT-116	IC₅₀	2.5 μM Compound: 14h	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
HT-29	IC₅₀	4.3 μM Compound: 14h	Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
MCF7	IC₅₀	2.5 μM Compound: 14h	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
MDA-MB-231	IC₅₀	2.4 μM Compound: 14h	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
NCI/ADR-RES	IC₅₀	33.7 μM Compound: 14h	Antiproliferative activity against human NCI-ADR-RES cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human NCI-ADR-RES cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
SK-OV-3	IC₅₀	29.9 μM Compound: 14h	Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
SW-620	IC₅₀	4.5 μM Compound: 14h	Antiproliferative activity against human SW-620 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human SW-620 cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]
T47D	IC₅₀	2.4 μM Compound: 14h	Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 24 hrs by sulforhodamine B assay	[PMID: 34847409]

In Vitro

Carbonic anhydrase inhibitor 12 (Compound 14h) (10 μM; 48 hours; single) has great influence on the specificity and the inhibitory potency against the different cancer cell lines^[1].
Carbonic anhydrase inhibitor 12 (2.4 μM in MCF-7 and 2.5 μM MDA-MB-231; 48 hours) can arrest the cell cycle of MCF-7 and MDA-MB-231 cells at the G2/M phase^[1].
Carbonic anhydrase inhibitor 12 (2.4 μM in MCF-7 and 2.5 μM MDA-MB-231; 48 hours) demonstrates an increase in the early apoptotic and late apoptotic phase in comparison to the control untreated cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:

MCF-7, T47D, MDA-MB-231, COLO 205, HCT-116, HT29, SW-620, A549, SK-OV-3 and NCI-ADR-RES^[1]
Concentration:

10 μM
Incubation Time:

48 hours
Result:

Showed a mean growth inhibitory activity of 55.12% and a broad range of antiproliferative activity against most of the tested cancer cell lines.

Cell Cycle Analysis

Cell Line:

MCF-7 and MDA-MB-231^[1]
Concentration:

2.4 μM in MCF-7, 2.5 μM MDA-MB-231
Incubation Time:

48 hours
Result:

Arrested the cell cycle of MCF-7 and MDA-MB-231 cells at the G2/M phase.

Apoptosis Analysis

Cell Line:

MCF-7 and MDA-MB-231^[1]
Concentration:

2.4 μM in MCF-7, 2.5 μM MDA-MB-231
Incubation Time:

48 hours
Result:

Demonstrated an increase in the early apoptotic and late apoptotic phase in comparison to the control untreated cells.

Chemical Information

CAS No. 2883451-38-3
Molecular Weight 640.53
Formula C₂₇H₂₂BrN₅O₅S₂
SMILES

COC1=CC=C(C(C2=NC(SCC(C3=CC=C(C=C3)Br)=O)=NC(NC4=CC=C(C=C4)S(N)(=O)=O)=C2C#N)=C1)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Abdel-Mohsen HT, et al. Application of the dual-tail approach for the design and synthesis of novel Thiopyrimidine-Benzenesulfonamide hybrids as selective carbonic anhydrase inhibitors. Eur J Med Chem. 2022;228:114004. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Carbonic anhydrase inhibitor 12

All Carbonic Anhydrase Isoforms