CAXII-IN-4
CAXII-IN-4 is a human carbonic anhydrase XII (hCA XII) inhibitor with Ki values of 4.9, 3079, 62.5 and 58.4 nM against hCA XII, hCA I, hCA II and hCA IX, respectively, showing high selectivity toward tumor-associated hCA XII. CAXII-IN-4 binds to the active site of hCA XII via a classic sulfamide binding mode, coordinates with Zn2+, forms a hydrogen bond with Ser133, and stabilizes the enzyme complex to reduce structural deviation. CAXII-IN-4 possesses both lipophilicity and polarity, and is predicted to have superior membrane permeability and oral absorbability. CAXII-IN-4 can be used in studies related to tumor-associated carbonic anhydrase inhibition.
For research use only. We do not sell to patients.
- Formula: C17H13BrN2O3S
- Molecular Weight:405.27
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
hCA IX 4.9 nM (Ki) |
hCA I 3079 nM (Ki) |
hCA II 62.5 nM (Ki) |
hCA IX 58.4 nM (Ki) |
In Vitro
CAXII-IN-4 (15j) (0.01-10 mM; 15 min) potently and selectively inhibits the tumor-associated human carbonic anhydrase XII isoform, with a Ki value of 4.9 nM, while exhibiting much weaker activity against hCA I, hCA II and hCA IX[1].
CAXII-IN-4 has predictable physicochemical properties, which indicate its favorable membrane permeability and potential for oral absorption compared with related compounds and the reference inhibitor acetazolamide[1].
CAXII-IN-4 binds to the active site of hCA XII via classic Zn2+ coordination and an additional hydrogen bond with Ser133, forming a stable and selective binding conformation[1].
CAXII-IN-4 forms a stable complex with hCA XII, which is evidenced by reduced structural fluctuations (lower RMSD) and persistent active site interactions during 50 ns molecular dynamics simulations[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 405.27
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Formula C17H13BrN2O3S
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SMILES
O=S(C1=CC=C(C(/C=C/C2=CNC3=C2C=CC(Br)=C3)=O)C=C1)(N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CAXII-IN-4 Related Classifications
HY-187173 Related Classifications
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