CellTracker Red CMTPX
Based on 3 publication(s) in Google Scholar
CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm).
For research use only. We do not sell to patients.
- Purity : 97.55%
- Formula: C42H40ClN3O4
- Molecular Weight:686.24
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) CellTracker Red CMTPX
More
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Stock Solution Preparation: Dissolve CellTracker Red CMTPX in high-quality anhydrous DMSO to create a 10 mM stock solution.
2. Working Solution Preparation: Dilute the stock solution 1:1000 to 1:2000 in pre-warmed (37°C), serum-free media (e.g., 5 μM final concentration).
3. Labeling (Suspension/Adherent Cells):
(1) Remove medium and add the pre-warmed working solution.
(2) Incubate cells for 15-45 minutes at 37°C.
(3) Remove working solution and replace with fresh pre-warmed media.
(4) Allow cells to incubate for another 30-60 minutes to allow the dye to convert to its membrane impermeant form.
4. Imaging: Use rhodamine or Texas Red filters (approx. 586/614 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Emission (Em)
614
Excitation (Ex)
586
Chemical Information
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Appearance Solid
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Molecular Weight 686.24
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Formula C42H40ClN3O4
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Color Blue to dark blue
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SMILES
CC1=CC(C)(C)N(C)C2=CC3=C(C(C4=C(C([O-])=O)C=CC(NC(C5=CC=C(CCl)C=C5)=O)=C4)=C6C=C7C(C)=CC(C)(C)[N+](C)=C7C=C6O3)C=C12.CC8=CC(C)(C)N(C)C9=CC%10=C(C(C%11=C(C([O-])=O)C=C(NC(C%12=CC=C(CCl)C=C%12)=O)C=C%11)=C%13C=C%14C(C)=CC(C)(C)[N+](C)=C%14C=C%13O%10)C=C89
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Free Radic Biol Med
Natural Linoleic Acid from Marine Fungus Eutypella sp. F0219 Blocks KEAP1/NRF2 Interaction and Ameliorates MASLD by Targeting FABP4. [Abstract]2024 Nov 1:224:630-643. PMID: 39299527
CellTracker Red CMTPX purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 1:224:630-643. [Abstract]
The live cell imaging probe CellTracker Red CMTPX (1 μg/mL; 37 ℃; 30 min) was used to evaluate the hepatoprotective efficacies of 61 marine-derived natural compounds on palmitic acid (PA)-treated hepatocytes.
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Phytochemistry
2026 Apr 27:114941. PMID: 42055272 -
CellTracker Red CMTPX purchased from MedChemExpress. Usage Cited in: bioRxiv. 2026 Jan 22.
Confocal microscopy analysis of Jurkat T cells pre-stained with CellTracker Red CMTPX (30 min).
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (145.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4572 mL | 7.2861 mL | 14.5722 mL | 36.4304 mL |
| 5 mM | 0.2914 mL | 1.4572 mL | 2.9144 mL | 7.2861 mL | |
| 10 mM | 0.1457 mL | 0.7286 mL | 1.4572 mL | 3.6430 mL | |
| 15 mM | 0.0971 mL | 0.4857 mL | 0.9715 mL | 2.4287 mL | |
| 20 mM | 0.0729 mL | 0.3643 mL | 0.7286 mL | 1.8215 mL | |
| 25 mM | 0.0583 mL | 0.2914 mL | 0.5829 mL | 1.4572 mL | |
| 30 mM | 0.0486 mL | 0.2429 mL | 0.4857 mL | 1.2143 mL | |
| 40 mM | 0.0364 mL | 0.1822 mL | 0.3643 mL | 0.9108 mL | |
| 50 mM | 0.0291 mL | 0.1457 mL | 0.2914 mL | 0.7286 mL | |
| 60 mM | 0.0243 mL | 0.1214 mL | 0.2429 mL | 0.6072 mL | |
| 80 mM | 0.0182 mL | 0.0911 mL | 0.1822 mL | 0.4554 mL | |
| 100 mM | 0.0146 mL | 0.0729 mL | 0.1457 mL | 0.3643 mL |
CellTracker Red CMTPX Related Classifications
HY-D1727 Related Classifications
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