N-Methylhydantoin
Based on 1 Customer Validation
N-Methylhydantoin is a creatinine degradation metabolite and non-substrate for microbial degradation.N-Methylhydantoin resists breakdown by most tested cytosine deaminase-forming microorganisms, which lack N-methylhydantoin amidohydrolase and N-carbamoylsarcosine amidohydrolase activity.N-Methylhydantoin forms as a product of creatinine hydrolysis by cytosine deaminase/creatinine deiminase in various microorganisms.
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- Reinheit : 99.81%
- CAS. Nr.: 616-04-6
- Formel: C4H6N2O2
- Molecular Weight:114.10
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
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Human Endogenous Metabolite |
Microbial Metabolite |
In Vitro
N-Methylhydantoin (0.5%; 1 day) cultivation of Pseudomonas putida 77 produces a creatinine deiminase/cytosine deaminase enzyme that is structurally and functionally identical to the enzyme produced under other cultivation conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 616-04-6
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Appearance Solid
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Molecular Weight 114.10
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Formel C4H6N2O2
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Color White to off-white
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SMILES
O=C1NC(CN1C)=O
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Synonyms
1-Methylhydantoin
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (876.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 8.7642 mL | 43.8212 mL | 87.6424 mL | 219.1061 mL |
| 5 mM | 1.7528 mL | 8.7642 mL | 17.5285 mL | 43.8212 mL | |
| 10 mM | 0.8764 mL | 4.3821 mL | 8.7642 mL | 21.9106 mL | |
| 15 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL | 14.6071 mL | |
| 20 mM | 0.4382 mL | 2.1911 mL | 4.3821 mL | 10.9553 mL | |
| 25 mM | 0.3506 mL | 1.7528 mL | 3.5057 mL | 8.7642 mL | |
| 30 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL | 7.3035 mL | |
| 40 mM | 0.2191 mL | 1.0955 mL | 2.1911 mL | 5.4777 mL | |
| 50 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL | 4.3821 mL | |
| 60 mM | 0.1461 mL | 0.7304 mL | 1.4607 mL | 3.6518 mL | |
| 80 mM | 0.1096 mL | 0.5478 mL | 1.0955 mL | 2.7388 mL | |
| 100 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1911 mL |
N-Methylhydantoin Related Classifications
HY-113382 Related Classifications
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Keywords
- N-Methylhydantoin
- 616-04-6
- 1-Methylhydantoin
- Endogenous Metabolite
- cytosine deaminase-forming microorganisms
- Pseudomonas putida 77
- N-carbamoylsarcosine amidohydrolase
- creatinine
- N-methylhydantoin amidohydrolase
- creatinine deiminase/cytosine deaminase enzyme
- microorganisms
- cytosine deaminase/creatinine deiminase
- Inhibitor
- inhibitor
- inhibit