Dodecyl azide
Based on 1 Customer Validation
Dodecyl azide (1-Azidododecane) is an azide group with a 12-carbon tail. Azide (N3) group can react with alkyne, BCN, DBCO via Click Chemistry.
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- Reinheit : 98.0%
- CAS. Nr.: 13733-78-3
- Formel: C12H25N3
- Molecular Weight:211.35
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Speicherung:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
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Help & FAQs
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Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 13733-78-3
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Appearance Liquid
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Molecular Weight 211.35
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Formel C12H25N3
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Color Colorless to light yellow
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SMILES
[N-]=[N+]=NCCCCCCCCCCCC
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Synonyms
1-Azidododecane
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (473.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
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Data Sheet (236 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7315 mL | 23.6574 mL | 47.3149 mL | 118.2872 mL |
| 5 mM | 0.9463 mL | 4.7315 mL | 9.4630 mL | 23.6574 mL | |
| 10 mM | 0.4731 mL | 2.3657 mL | 4.7315 mL | 11.8287 mL | |
| 15 mM | 0.3154 mL | 1.5772 mL | 3.1543 mL | 7.8858 mL | |
| 20 mM | 0.2366 mL | 1.1829 mL | 2.3657 mL | 5.9144 mL | |
| 25 mM | 0.1893 mL | 0.9463 mL | 1.8926 mL | 4.7315 mL | |
| 30 mM | 0.1577 mL | 0.7886 mL | 1.5772 mL | 3.9429 mL | |
| 40 mM | 0.1183 mL | 0.5914 mL | 1.1829 mL | 2.9572 mL | |
| 50 mM | 0.0946 mL | 0.4731 mL | 0.9463 mL | 2.3657 mL | |
| 60 mM | 0.0789 mL | 0.3943 mL | 0.7886 mL | 1.9715 mL | |
| 80 mM | 0.0591 mL | 0.2957 mL | 0.5914 mL | 1.4786 mL | |
| 100 mM | 0.0473 mL | 0.2366 mL | 0.4731 mL | 1.1829 mL |
Dodecyl azide Related Classifications
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