Cat. No.: HY-N2584A

Isoxanthohumol

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Based on 4 publication(s) in Google Scholar

Isoxanthohumol is an orally active flavonoid compound. Isoxanthohumol has biological activities such as anti-tumor, anti-inflammatory, antioxidant, antiviral, antifungal, and inhibition of adipogenesis. Isoxanthohumol can induce apoptosis, autophagy, and migration of tumor cells. Isoxanthohumol is active against viruses such as HSV, BVDV, CMV, and Rhino. Isoxanthohumol can be used for the research of tumors, metabolic, and inflammatory diseases.

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Pureté: 99.91%
CAS No.: 521-48-2
Formule: C₂₁H₂₂O₅
Masse moléculaire:354.40

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Isoxanthohumol Chemical Structure

CAS No.: 521-48-2

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Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Bioz

Activité biologique

Description

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Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	IC₅₀	88.82 μM Compound: 3	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 23434138]
Ishikawa	IC₅₀	24.9 μM Compound: IX	Cytotoxicity against human Ishikawa cells after 96 hrs by SRB assay Cytotoxicity against human Ishikawa cells after 96 hrs by SRB assay	[PMID: 28812892]
MCF7	IC₅₀	15.3 μM Compound: IX	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 2 days by SRB assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 2 days by SRB assay	[PMID: 33257172]
MCF7	IC₅₀	26.54 μM Compound: 3	Cytotoxicity against human MCF7 cells after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells after 72 hrs by SRB assay	[PMID: 23434138]
MCF7	IC₅₀	4.69 μM Compound: IX	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 4 days by SRB assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 4 days by SRB assay	[PMID: 33257172]
PC-3	IC₅₀	71.32 μM Compound: 3	Cytotoxicity against human PC3 cells after 72 hrs by SRB assay Cytotoxicity against human PC3 cells after 72 hrs by SRB assay	[PMID: 23434138]

In Vitro

Isoxanthohumol (0-100 μM; 20 min-6 d) reduces the viability, induces apoptosis of mature 3T3-L1 adipocytes, and inhibits the differentiation of 3T3-L1 preadipocytes. The mechanisms are associated with ROS generation and mitochondrial dysfunction^[1].
Isoxanthohumol (2.5-50 µg/mL) inhibits Botrytis cinerea, Fusarium graminearum, and Sclerotinia sclerotiorum with EC₅₀ values of 4.32, 16.50, and 14.52 µg/mL, respectively^[2].
Isoxanthohumol (1.6-100 μM; 0-7 d) suppresses the proliferation, adhesion, migration, invasion, and colony formation, induces apoptosis and autophagy, and downregulates the integrin signaling pathway in B16-F10 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Mature 3T3-L1 adipocytes
Concentration:

0, 25, 50, 75 and 100 μM
Incubation Time:

24 and 48 h
Result:

Inhibited the cell viability in a dose-dependent manner.

Western Blot Analysis^[3]

Cell Line:

B16-F10 cells
Concentration:

30 μM
Incubation Time:

0, 2, 6, 24 and 48 h
Result:

Inhibited the levels of integrin α-6、FAK、vinculin、Rho and α-SMA.

In Vivo

Isoxanthohumol (20 mg/kg; oral administration; 18 days) exhibits antimetastatic activity in a B16-F10 mouse tumor model^[3].
Isoxanthohumol (60 mg/kg; oral gavage; 8 days) shows ameliorative effects in a DSS (HY-116282C)-induced mouse colitis model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice treated B16-F10 cells^[3]
Dosage:

20 mg/kg
Administration:

Oral administration; 18 days
Result:

Reduced the number and diameter of lung metastatic nodules.
Significantly decreased the expression of HMGB1 in lung metastatic cells, while enhanced the expression of S100 protein, and decreased the expression of the proliferation marker Ki-67, indicating the differentiation of metastatic cells to a less invasive phenotype.
Caused no significant toxicity.

Animal Model:

Male SPF C57BL/6 mice aged 6-8 weeks old treated DSS (HY-116282C)^[4]
Dosage:

60 mg/kg
Administration:

Oral gavage; 8 days
Result:

Significantly attenuated DSS-induced colitis, evidenced by reduced DAI scores and histological improvements.
Suppressed pro-inflammatory Th17/Th1 cells, promoted anti-inflammatory Treg cells. Restored gut microbiota disorder, increased gut microbiota diversity.
Upregulated D-(+)-mannose and L-threonine, and regulated pyruvate metabolic pathway.

Chemical Information

CAS No. 521-48-2
Appearance Solid
Masse moléculaire 354.40
Formule C₂₁H₂₂O₅
Color Off-white to light yellow
SMILES

OC1=CC(OC)=C2C(CC(OC2=C1C/C=C(C)\C)C3=CC=C(C=C3)O)=O
Structure Classification
- Flavonoids
- Flavonones
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

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Journal Impact Factor

Phytother Res

Xanthohumol inhibits non-small cell lung cancer via directly targeting T-lymphokine-activated killer cell-originated protein kinase. [Abstract]

2023 Jul;37(7):3057-3068. PMID: 36882184
Int J Mol Sci

Dual Targeting of HIF-1α and DLL4 by Isoxanthohumol Potentiates Immune Checkpoint Blockade. [Abstract]

2026 Feb 5;27(3):1576. PMID: 41683994
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
bioRxiv

2025 Jan 03.

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (141.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8217 mL

14.1084 mL

28.2167 mL

5 mM

0.5643 mL

2.8217 mL

5.6433 mL

10 mM

0.2822 mL

1.4108 mL

2.8217 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (7.05 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Yang JY, et al. Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis. Apoptosis. 2007 Nov;12(11):1953-63. [Content Brief]

[2]. Yan YF, et al. The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn. Int J Mol Sci. 2021 Oct 7;22(19):10853. [Content Brief]

[3]. Krajnović T, et al. The hop-derived prenylflavonoid isoxanthohumol inhibits the formation of lung metastasis in B16-F10 murine melanoma model. Food Chem Toxicol. 2019 Jul;129:257-268. [Content Brief]

[4]. Yang YN, et al. Therapeutic effects and mechanisms of isoxanthohumol on DSS-induced colitis: regulating T cell development, restoring gut microbiota, and improving metabolic disorders. Inflammopharmacology. 2024 Jun;32(3):1983-1998. [Content Brief]

[5]. Żołnierczyk AK, et al. Isoxanthohumol--Biologically active hop flavonoid. Fitoterapia. 2015 Jun;103:71-82. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8217 mL	14.1084 mL	28.2167 mL	70.5418 mL
	5 mM	0.5643 mL	2.8217 mL	5.6433 mL	14.1084 mL
	10 mM	0.2822 mL	1.4108 mL	2.8217 mL	7.0542 mL
	15 mM	0.1881 mL	0.9406 mL	1.8811 mL	4.7028 mL
	20 mM	0.1411 mL	0.7054 mL	1.4108 mL	3.5271 mL
	25 mM	0.1129 mL	0.5643 mL	1.1287 mL	2.8217 mL
	30 mM	0.0941 mL	0.4703 mL	0.9406 mL	2.3514 mL
	40 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7635 mL
	50 mM	0.0564 mL	0.2822 mL	0.5643 mL	1.4108 mL
	60 mM	0.0470 mL	0.2351 mL	0.4703 mL	1.1757 mL
	80 mM	0.0353 mL	0.1764 mL	0.3527 mL	0.8818 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7054 mL

Isoxanthohumol

4 Cited Publications

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Isoxanthohumol

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Publications Citing Use of MedChemExpress (MCE) Isoxanthohumol

Voir tous les produits spécifiques à Isoform HSV

Voir tous les produits spécifiques à Isoform Integrin

Activité biologique

Chemical Information

Publications (4)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Isoxanthohumol Related Classifications

Cat. No.: HY-N2584A