Mouse Laminin
Based on 1 Customer Validation
Mouse Laminin is a Laminin from mouse that can be used as a thin coating on tissue culture surfaces or as a soluble additive to culture media.
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- Pureté: 90%
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Black, clear bottom 96-well tissue culture plates are coated with either Laminin-entactin (LE), Laminin, or extracellular matrix (ECM) gel at a concentration range of 25-200 µg/mL. Each adhesive substrate is diluted in PBS (6 mM KCl, 1 mM Na2HPO4, 0.2 mM, NaH2HPO4.H20, 1.4 mM MgSO4.7H20, 128 mM NaCl, 1.2 mM Ca2+, 10 mM HEPES, pH 7.4) to obtain the required final concentrations (25, 50, 75, 100, 125, 150, 175 and 200 μg/mL), and 5 µL is placed in the middle of each well. The coated plates are incubated overnight at 5% CO2 and 37˚С, washed twice with PBS buffer and air dried in a laminar flow hood prior to seeding of the cardiomyocytes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Mouse Laminin]
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Livraison
Shipping with dry ice.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Siano G, et al. Gene Expression of Disease-related Genes in Alzheimer's Disease is Impaired by Tau Aggregation. J Mol Biol. 2020 Dec 4;432(24):166675. [Content Brief]
[2]. Engvall E, et al. Laminin from rat yolk sac tumor: isolation, partial characterization, and comparison with mouse laminin. Arch Biochem Biophys. 1983 Apr 15;222(2):649-56. [Content Brief]
[3]. Lumkwana D, et al. Laminin, laminin-entactin and extracellular matrix are equally appropriate adhesive substrates for isolated adult rat cardiomyocyte culture and experimentation. Cell Adh Migr. 2018;12(5):503-511. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mouse Laminin Related Classifications
HY-NP139 Related Classifications
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